Human Anti-E. histolytica Recombinant Antibody (clone B220) (CAT#: HPAB-2042-FY)

This product is a recombinant human antibody that recognizes E. histolytica. The monoclonal human antibody B220 reacts with the epitope of E. histolytica.


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FC

Figure 1 Gal/GalNAc lectin-mediated protection is adoptively transferred by T cells, not serum antibodies. Purified Gal/GalNAc lectin-immunized (closed bars) and PBS sham-immunized (open bars) CBA mice were challenged with E. histolytica trophozoites 4 weeks after the final boost.

Figure 1 Gal/GalNAc lectin-mediated protection is adoptively transferred by T cells, not serum antibodies. Purified Gal/GalNAc lectin-immunized (closed bars) and PBS sham-immunized (open bars) CBA mice were challenged with E. histolytica trophozoites 4 weeks after the final boost.

Splenocytes and mesenteric lymph node cells were isolated from lectin-immunized or PBS sham-immunized mice and enriched for CD3+ T cells. The purity of enriched cells was determined by fluorescence-activated cell sorter staining for CD3 and B220 before and after enrichment.

Guo, X., Barroso, L., Becker, S. M., Lyerly, D. M., Vedvick, T. S., Reed, S. G., ... & Houpt, E. R. (2009). Protection against intestinal amebiasis by a recombinant vaccine is transferable by T cells and mediated by gamma interferon. Infection and immunity, 77(9), 3909-3918.


Specifications

  • Host Species
  • Human
  • Type
  • Human IgG, κ
  • Specificity
  • E. histolytica
  • Species Reactivity
  • E. histolytica
  • Clone
  • B220
  • Applications
  • IF, WB, FC
  • Related Disease
  • E. histolytica

Product Property

  • Purity
  • >95% as determined by SDS-PAGE analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The E. histolytica antibody has been reported in applications of Immunofluorescence, Western Blot, Flow Cytometry.

Target

  • Alternative Names
  • Entamoeba histolytica; E. histolytica

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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