CytoStream™ Rabbit Anti-EEF1G Recombinant Antibody (VS13-YC338)
CAT#: VS13-YC338
The CytoStream™ Rabbit Anti-EEF1G Recombinant Antibody is specific for human, mouse and rat EEF1G. EEF1G functions in the elongation factor-1 complex, integrating subunit complexes impacting the aminoacyl-tRNA binding dynamics during translation. The anti-EEF1G antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55316/1050_E1_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40688/102362_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40688/102362_A_7_3.jpg
Normal Tissue
Figure 4 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40688/102362_A_6_6.jpg
Normal Tissue
Figure 5 Fallopian tube
Ciliated cells
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40688/102362_A_8_7.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40688/102362_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000254772-EEF1G
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Specifications
- Immunogen
- Recombinant human EEF1G.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EEF1G
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/50
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Eukaryotic Translation Elongation Factor 1 Gamma; EEF-1B Gamma; EF-1-Gamma; EF1G; Translation Elongation Factor EEF-1 Gamma Chain; Pancreatic Tumor-Related Protein; Elongation Factor 1-Gamma; PRO1608; GIG35;
- Gene ID
- 1937
- UniProt ID
- P26641
- Long Name
- Eukaryotic Translation Elongation Factor 1 Gamma
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "EEF1G"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2360MZ | Recombinant Mouse Anti-Human EEF1G Antibody | ICC, IF, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1095 | Hi-Affi™ Rabbit Anti-EEF1G Recombinant Antibody (clone DS1095AB) | WB, IHC, ICC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2165 | Anti-EEF1G Immunohistochemistry Kit | IHC | |
| VS-0525-XY2166 | Anti-Mouse EEF1G Immunohistochemistry Kit | IHC |
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