Rabbit Anti-EEF2 Recombinant Antibody (clone R07-2C0) (CAT#: VS3-FY1999)
The R07-2C0 recombinant monoclonal antibody recognizes human EEF2. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We validate the QC result of the antibody using western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). The binding specificity of the elongation factor 2 antibody was determined using western blot analysis of EEF2/Elongation factor 2 in 3T3, MEF lysates and western blot analysis of EEF2/Elongation factor 2 in A549, U2OS, C6 lysates using EEF2/Elongation factor 2 antibody.
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WB
Figure 1 Anti-EEF2 Antibody (VS3-FY1999) in WB.
The EEF2/Elongation factor 2 expression in 3T3, MEF lysates were detected using the anti-EEF2/Elongation factor 2 by western blot assay.
WB
Figure 2 Anti-EEF2 Antibody (VS3-FY1999) in WB.
The EEF2/Elongation factor 2 expression in A549, U2OS, C6 lysates were detected using the anti-EEF2/Elongation factor 2 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human EEF2/elongation factor 2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EEF2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-2C0
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 95 kDa, while the observed molecular weight stands at 95 kDa.
- Related Disease
- Spinocerebellar Ataxia 26 and Diphtheria. Among its related pathways are Peptide chain elongation and Innate Immune System
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Eukaryotic Translation Elongation Factor 2; Polypeptidyl-TRNA Translocase; EF-2; EF2; Elongation Factor 2; EEF-2; SCA26
- Gene ID
- 1938
- UniProt ID
- P13639
- Sequence Similarities
- Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase.
Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment.
(P13639-EF2_HUMAN)
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine (). (P13639-EF2_HUMAN)
(Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis. (P13639-EF2_HUMAN)
ISGylated. (P13639-EF2_HUMAN)
Proteolytically processed at two sites following phosphorylation by CSK. (P13639-EF2_HUMAN)
SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage. (P13639-EF2_HUMAN)
Ubiquitination at Lys32, Lys42, Lys152, Lys159, Lys235, Lys239, Lys272, Lys275, Lys318, Lys333, Lys366, Lys386, Lys426, Lys481, Lys498, Lys512, Lys594, Lys638, Lys648, Lys688, and Lys845 (NX_P13639 [NX_P13639-1])
Modification sites at PhosphoSitePlus (P13639)
Glycosylation from GlyGen (P13639) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- NP_001952.1
- Function
- Catalyzes the GTP-dependent ribosomal translocation step during translation elongation.
During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Immunotoxins
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G017E | Anti-EEF2 immunotoxin G4S (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G017D | Anti-EEF2 immunotoxin G4S (scFv)-DT | Cytotoxicity assay, Function study |
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