Rabbit Anti-EEF2 Recombinant Antibody (clone R07-2C0) (CAT#: VS3-FY1999)
The R07-2C0 recombinant monoclonal antibody recognizes human EEF2. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We validate the QC result of the antibody using western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). The binding specificity of the elongation factor 2 antibody was determined using western blot analysis of EEF2/Elongation factor 2 in 3T3, MEF lysates and western blot analysis of EEF2/Elongation factor 2 in A549, U2OS, C6 lysates using EEF2/Elongation factor 2 antibody.
Specific Inquiry
WB
Figure 1 Anti-EEF2 Antibody (VS3-FY1999) in WB.
The EEF2/Elongation factor 2 expression in 3T3, MEF lysates were detected using the anti-EEF2/Elongation factor 2 by western blot assay.
WB
Figure 2 Anti-EEF2 Antibody (VS3-FY1999) in WB.
The EEF2/Elongation factor 2 expression in A549, U2OS, C6 lysates were detected using the anti-EEF2/Elongation factor 2 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human EEF2/elongation factor 2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EEF2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-2C0
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 95 kDa, while the observed molecular weight stands at 95 kDa.
- Related Disease
- Spinocerebellar Ataxia 26 and Diphtheria. Among its related pathways are Peptide chain elongation and Innate Immune System
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Eukaryotic Translation Elongation Factor 2; Polypeptidyl-TRNA Translocase; EF-2; EF2; Elongation Factor 2; EEF-2; SCA26
- Gene ID
- 1938
- UniProt ID
- P13639
- Sequence Similarities
- Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase.
Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment.
(P13639-EF2_HUMAN)
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine (). (P13639-EF2_HUMAN)
(Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis. (P13639-EF2_HUMAN)
ISGylated. (P13639-EF2_HUMAN)
Proteolytically processed at two sites following phosphorylation by CSK. (P13639-EF2_HUMAN)
SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage. (P13639-EF2_HUMAN)
Ubiquitination at Lys32, Lys42, Lys152, Lys159, Lys235, Lys239, Lys272, Lys275, Lys318, Lys333, Lys366, Lys386, Lys426, Lys481, Lys498, Lys512, Lys594, Lys638, Lys648, Lys688, and Lys845 (NX_P13639 [NX_P13639-1])
Modification sites at PhosphoSitePlus (P13639)
Glycosylation from GlyGen (P13639) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- NP_001952.1
- Function
- Catalyzes the GTP-dependent ribosomal translocation step during translation elongation.
During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "EEF2"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2641z | Mouse Anti-EEF2 Recombinant Antibody (clone 26G3) | WB, ELISA, ICC, IF, IHC | Mouse IgG1 |
| MOB-2361MZ | Recombinant Mouse Anti-Human EEF2 Antibody (clone FMM3B22I3) | WB | Mouse antibody |
| VS3-QX337 | Mouse Anti-EEF2 Recombinant Antibody (clone 4B3-G7-H5) | WB, ICC | Mouse IgG2b |
| VS3-QX338 | Mouse Anti-EEF2 Recombinant Antibody (clone 6E12-G5-C8) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-WK160 | Mouse Anti-EEF2 Recombinant Antibody (clone H1-B6) | WB, ELISA | Mouse IgG1 |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G017E | Anti-EEF2 immunotoxin G4S (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G017D | Anti-EEF2 immunotoxin G4S (scFv)-DT | Cytotoxicity assay, Function study |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1096 | Hi-Affi™ Rabbit Anti-EEF2 Recombinant Antibody (clone DS1096AB) | WB, IHC-P, ICC, IF | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1929 | PE-A*02:01/Human EEF2 (ILTDITKGV) MHC Tetramer | FCM | |
| MHC-LC4409 | PE-A*01:01/Human EEF2 (YFDPANGKF) MHC Tetramer | FCM |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-FY1999. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.