Mouse Anti-EFNB3 Recombinant Antibody (VS-0224-XY39)
CAT#: VS-0224-XY39
The product is a mouse antibody directed against mouse, rat and human ephrin-B3 protein. It was purified by protein A. This antibody has been detected using Enzyme-linked immunosorbent assay, Western blot, Immunofluorescence and Immunoprecipitation.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in subset of neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1623/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1623/16222_B_7_5.jpg
Normal Tissue
Figure 3 Hippocampus
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1623/16222_B_8_6.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1623/15988_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1623/15988_A_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (AF22, AN3-CA, hTERT-RPE1, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108947-EFNB3
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a, kappa
- Specificity
- Human EFNB3
- Species Reactivity
- Human
- Applications
- ELISA, Western Blot, Immunofluorescence, Immunoprecipitation
- Related Disease
- Nipah Virus Encephalitis; Cortical Dysplasia, Complex
Product Property
- Purification
- Protein A purified
- Buffer
- PBS
- Preservative
- 0.1% sodium azide and 0.1% gelatin
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested in ELISA (1/30-1/3000), Western blot (1/100-1/1000) and Immunofluorescence (1/50-1/500).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "EFNB3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2655z | Mouse Anti-EFNB3 Recombinant Antibody (MOB-2655z) | WB | Mouse IgG2b |
| MOB-0677CT | Recombinant Mouse anti-Human EFNB3 Monoclonal antibody (NN0375- 23Y2) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2655z-F(E) | Recombinant Anti-Human EFNB3 Antibody Fab Fragment | ELISA, FacS, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2655z-S(P) | Recombinant Anti-Human EFNB3 Antibody scFv Fragment | IF, Neut, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC44 | Recombinant Anti-EFNB3 Vesicular Antibody, EV Displayed (VS-0425-YC44) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2177 | Anti-EFNB3 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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