Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-710)

Name: Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-710)
SKU: AFC-TAB-710
Rating: 5 (1 reviews)

CAT#: AFC-TAB-710

This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant humanized (from mouse) antibody expressed in CHO binding to human EGFR. It is a humanized monoclonal antibody.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Normal Tissue Figure 7 Placenta

Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Normal Tissue Figure 8 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (A-431)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Humanized (from mouse)

Type
ADCC enhanced antibody

Species Reactivity
Human

Applications
ELISA, IP, FC, FuncS, Neut, IF

Conjugate
Unconjugated

Trade name
Theracim

Generic Name
Nimotuzumab

Related Disease
Glioma cancers

Product Property

Purity
>95% by SDS-PAGE

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), ERBB; ERBB1;

Gene ID
1956

UniProt ID
P00533

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Biosimilar Overview

Please refer to Nimotuzumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Nimotuzumab.

Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Pancreatic Cancer

Hepatocellular Carcinoma

Cushing Syndrome

Related Signaling Pathways

JAK-STAT Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AFC-TAB-710.pdf

MSDS

MSDS - AFC-TAB-710.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "nimotuzumab"

Anti-EGFR Recombinant Antibody (Nimotuzumab)
Recombinant humanized (from mouse) antibody expressed in CHO binding to human EGFR. Nimotuzumab is a humanized monoclonal antibody that as of 2014 had orphan status in the US and EU for glioma, and marketing approval in India, China, and other countries for squamous cell carcinomas of the head and neck.

DrugMonitor™ Anti-Nimotuzumab Antibody (VS-1224-YC808)
Nimotuzumab (h-R3), a humanized monoclonal antibody targeting EGFR, has shown promising efficacy combined with radiation in several cancer types, meriting further investigation. The DrugMonitor™ Anti-Nimotuzumab Antibody (VS-1224-YC808) is an anti-drug antibody (ADA) against Nimotuzumab. This drug-based antibody is raised in mice immunized with the Nimotuzumab. The anti-Nimotuzumab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Nimotuzumab in samples.

See other products for "EGFR"

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CAT	Product Name	Application	Type
TAB-750	Anti-EGFR/HER1 Recombinant Antibody (TAB-750)	Neut, ELISA, IF, IP, FuncS, FC, WB	IgG1 - kappa
TAB-272MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P))	FuncS	Mouse scFv
PABX-051	Recombinant Human Anti-EGFR Antibody (Matuzumab)	WB, Neut, FuncS, FC, ELISA, IF, Inhib	IgG
PABX-051-F (E)	Recombinant Mouse Anti-EGFR Antibody Fab Fragment (Matuzumab)	WB, Neut, FuncS	Fab
TAB-464CQ	Anti-Human EGFR Recombinant Antibody (TAB-464CQ)	ELISA, IHC, FC, IP, IF, FuncS	IgG1, κ

CAT	Product Name	Application	Type
TAB-165	Anti-Human EGFR Recombinant Antibody (Matuzumab)	Neut, ELISA, IF, IP, FuncS, FC, ICC	IgG1
TAB-710	Anti-EGFR Recombinant Antibody (Nimotuzumab)	ELISA, IP, FC, FuncS, Neut, IF, IHC	IgG1 - kappa
TAB-753	Anti-EGFR Recombinant Antibody (Imgatuzumab)	Neut, ELISA, IF, IP, FuncS, FC, WB	IgG1 - kappa
PABX-052	Recombinant Human Anti-EGFR Antibody (PABX-052)	WB, ELISA, FuncS, IB, FC, SPR, Apop	IgG
PABX-052-F (E)	Recombinant Human Anti-EGFR Antibody Fab Fragment (PABX-052-F (E))	WB, ELISA, FuncS	Fab

CAT	Product Name	Application	Type
PFBL-459	Human Anti-EGFR Recombinant Antibody (clone C225); Fab Fragment	FC	Human Fab
PFBL-460	Human Anti-EGFR Recombinant Antibody (clone h-R3); Fab Fragment	ELISA, WB, FuncS	Humanized Fab
HPAB-2194LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2194LY-F(E))	ELISA, WB	Mouse Fab
HPAB-2195LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2195LY-F(E))	ELISA	Mouse Fab
HPAB-2196LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2196LY-F(E))	ELISA	Mouse Fab

CAT	Product Name	Application	Type
PFBW-171	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (PFBW-171)	WB	Mouse Fab
HPAB-0010-YC-S(P)	Mouse Anti-EGFR Recombinant Antibody (clone L211C); scFv Fragment	ELISA, FC	Mouse scFv
HPAB-0011-YC-S(P)	Human Anti-EGFR Recombinant Antibody (clone Pep 1); scFv Fragment	FC	Human scFv
HPAB-0012-YC-S(P)	Human Anti-EGFR Recombinant Antibody (clone Pep 2); scFv Fragment	FC	Human scFv
HPAB-0013-YC-S(P)	Human Anti-EGFR Recombinant Antibody (clone Pep 3); scFv Fragment	FC	Human scFv

CAT	Product Name	Application	Type
TAB-0564CL-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0564CL-S(P))	ELISA	Mouse scFv
TAB-0565CL-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0565CL-S(P))	ELISA	Mouse scFv
TAB-301MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-301MZ-S(P))	ELISA	Mouse scFv
TAB-303MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-303MZ-S(P))	ELISA	Mouse scFv
TAB-304MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-304MZ-S(P))	ELISA	Mouse scFv

CAT	Product Name	Application	Type
TAB-270MZ	Human Anti-EGFR Recombinant Antibody (TAB-270MZ)	ELISA	Human antibody
TAB-278MZ	Human Anti-EGFR Recombinant Antibody (TAB-278MZ)	Cyt, ELISA, Inhib	Human IgG
TAB-279MZ	Human Anti-EGFR Recombinant Antibody (TAB-279MZ)	Cyt, ELISA, Inhib	Human IgG
TAB-282MZ	Human Anti-EGFR Recombinant Antibody (TAB-282MZ)	Cyt, FuncS, Inhib	Human IgG
TAB-283MZ	Human Anti-EGFR Recombinant Antibody (TAB-283MZ)	Cyt, FuncS, Inhib	Human IgG

CAT	Product Name	Application	Type
Gly-055LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)
Gly-144LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
Gly-055LC-1	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)

CAT	Product Name	Application	Type
Gly-167LC	Recombinant Anti-Human EGFR Antibody (Non-glycosylated)	ELISA	Human antibody

CAT	Product Name	Application	Type
BRD-0183MZ	Chicken Anti-EGFR Polyclonal IgY	WB	Chicken antibody

CAT	Product Name	Application	Type
MHC-LC773	A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer	FCM
MHC-LC4545	PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer	FCM

CAT	Product Name	Application	Type
NEUT-722CQ	Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011)	Neut	Rabbit IgG
NEUT-723CQ	Mouse Anti-EGFR Recombinant Antibody (clone CBL931)	WB, IP, IHC, ICC, Neut	Mouse IgG1
NEUT-724CQ	Rabbit Anti-EGFR Recombinant Antibody (NEUT-724CQ)	IF, FC, WB, IP, Neut	Rabbit IgG

CAT	Product Name	Application	Type
MOR-4520	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS)	IF, ICC, FC	Rabbit IgG
MOR-4570	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS)	ELISA	Rabbit IgG
MOR-4675	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS)	WB, IF, ICC, FC	Rabbit IgG
MOR-4676	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS)	WB, IF, ICC, FC	Rabbit IgG
MOR-0033-FY	Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004)	ICC, IHC, WB	Rabbit IgG

CAT	Product Name	Application	Type
FAMAB-0202-CN	Human Anti-EGFR Recombinant Antibody (clone E10)	ELISA, WB	Human IgG
HPAB-2194LY	Mouse Anti-EGFR Recombinant Antibody (HPAB-2194LY)	ELISA, WB	Mouse IgG
HPAB-2195LY	Mouse Anti-EGFR Recombinant Antibody (HPAB-2195LY)	ELISA	Mouse IgG
HPAB-2196LY	Mouse Anti-EGFR Recombinant Antibody (HPAB-2196LY)	ELISA	Mouse IgG
HPAB-2197LY	Mouse Anti-EGFR Recombinant Antibody (HPAB-2197LY)	ELISA	Mouse IgG

CAT	Product Name	Application	Type
AFC-TAB-165	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-165)	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody
AFC-TAB-464CQ	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-464CQ)	ELISA, IHC, FC, IP, IF, FuncS	ADCC enhanced antibody
AFC-TAB-003	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-003)	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody
AFC-TAB-040	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-040)	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody
AFC-TAB-119	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-119)	FC, IP, ELISA, Neut, FuncS, IF	ADCC enhanced antibody

CAT	Product Name	Application	Type
HPAB-AP881-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP881-YC)	ELISA, FC, IP, FuncS	Llama VHH
HPAB-AP882-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP882-YC)	ELISA	Llama VHH
HPAB-AP883-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP883-YC)	ELISA	Llama VHH
HPAB-AP884-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP884-YC)	ELISA	Llama VHH
HPAB-AP885-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP885-YC)	ELISA	Llama VHH

CAT	Product Name	Application	Type
VS-0424-XY84	AbPlus™ Anti-EGFR Magnetic Beads (pSEX81-6)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0924-YC32	Mouse Anti-EGFR Recombinant Antibody (VS-0924-YC32) - Cancer Stem Cell Marker	IHC, WB	Mouse IgG1
VS-0924-YC35	Rabbit Anti-EGFR Antibody (VS-0924-YC35) - Cancer Stem Cell Marker	IHC, WB, IF	Rabbit IgG

CAT	Product Name	Application	Type
VS-1024-XY177	Mouse Anti-NHP EGFR Recombinant Antibody (clone 225)	IF, IP	Mouse IgG1

CAT	Product Name	Application	Type
VS-0125-FY28	Human Anti-EGFR (clone ABT-806) scFv-Fc Chimera	FC, Cyt	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0225-XY102	CytoStream™ Mouse Anti-EGFR Recombinant Antibody (VS-0225-XY102)	FC	Mouse IgG1, kappa

CAT	Product Name	Application
VS-0325-XY735	Anti-EGFR Immunohistochemistry Kit	IHC
VS-0525-XY2183	Anti-Mouse EGFR Immunohistochemistry Kit	IHC
VS-0525-XY2182	Anti-Human EGFR Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0425-YC340	Recombinant Anti-EGFR Vesicular Antibody, EV Displayed (VS-0425-YC340)	ELISA, FC, Neut, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-YC65	Recombinant Anti-EGFR (AA 269-278 x AA 526-535) Biparatopic Antibody, Tandem scFv (Clone Pep 2 x Clone Pep 3)	FC	Tandem scFv
VS-0525-YC66	Recombinant Anti-EGFR (AA 582-591 x AA 606-614) Biparatopic Antibody, Tandem scFv (Clone Pep 4 x Clone Pep 1)	FC	Tandem scFv
VS-0525-YC68	Recombinant Anti-EGFR (AA 526-535 x AA 600-605) Biparatopic Antibody, Tandem scFv (Clone Pep 3 x Clone Pep 5)	FC	Tandem scFv
VS-0525-YC213	Recombinant Anti-EGFR (Domain II x Domain III) Biparatopic Antibody, Tandem scFv	ELISA, FC, IF, IHC, IP	Tandem scFv

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