Recombinant Human Anti-EGFR Antibody (13.1.2) (CAT#: HPAB-0161CQ)

This product is a recombinant human antibody that recognizes EGFR. The antibody binds to EGFRvIII and the epitope is EEKKGNY. The 13.1.2 antibody possess very high affinities for EGFRvIII, are internalized well by cells.

  • Published Data
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Epidermal growth factor receptor variant III (EGFRvIII) recognition by different antibodies.

Lysates of EGFRvIII‐transfected and parental A431 and HEK293T cells were analyzed by immunoblot using the stated antibodies for detection and β‐actin as a loading control. Arrows indicate the expected molecular weights of wild‐type (wt) EGFR and EGFRvIII, which appears as a double band. 13.1.2 exclusively detected EGFRvIII, whereas ch806 and MR1‐1 also bound to wt EGFR on non‐transfected cells.

Klausz, K., Berger, S., Lammerts van Bueren, J. J., Derer, S., Lohse, S., Dechant, M.,... & Valerius, T. (2011). Complement‐mediated tumor‐specific cell lysis by antibody combinations targeting epidermal growth factor receptor (EGFR) and its variant III (EGFRvIII). Cancer science, 102(10), 1761-1768.

FuncS

Figure 2 Epidermal growth factor receptor variant III (EGFRvIII) recognition by different antibodies.

Indirect immunofluorescence revealed a similar binding of EGFRvIII antibodies to EGFRvIII‐expressing A431 (A431‐vIII) and HEK293T (HEK293T‐vIII) cells (lower panel). Additional binding to A431 cells was obtained with MR1‐1 (grey line) and ch806 (black line). MR1‐1 also displayed reactivity with HEK293T cells, whereas 13.1.2 (dotted line) only bound to EGFRvIII‐transfected cells. Zalutumumab (black fill) recognized wt EGFR and EGFRvIII. An irrelevant IgG1 antibody (KLH) served as a control (grey fill).

Klausz, K., Berger, S., Lammerts van Bueren, J. J., Derer, S., Lohse, S., Dechant, M.,... & Valerius, T. (2011). Complement‐mediated tumor‐specific cell lysis by antibody combinations targeting epidermal growth factor receptor (EGFR) and its variant III (EGFRvIII). Cancer science, 102(10), 1761-1768.

FC

Figure 3 Epidermal growth factor receptor variant III (EGFRvIII) recognition by different antibodies.

Flow cytometric analyses relative to KLH binding (relative fluorescence intensity [RFI]; n = 3) yielded concentration‐dependent antibody binding to HEK293T‐vIII cells with the lowest EC50 value and a 95% confidence interval (CI) for zalutumumab (•), similar values for ch806 (△) and 13.1.2 (♦), and highest values for MR1‐1 (▼).

Klausz, K., Berger, S., Lammerts van Bueren, J. J., Derer, S., Lohse, S., Dechant, M.,... & Valerius, T. (2011). Complement‐mediated tumor‐specific cell lysis by antibody combinations targeting epidermal growth factor receptor (EGFR) and its variant III (EGFRvIII). Cancer science, 102(10), 1761-1768.

Block

Figure 4 Crossblocking experiments and C1q deposition.

MR1-1 and 13.1.2 crossblocked each other, indicating an overlapping epitope, while ch806 did not compete with any other antibody.

Klausz, K., Berger, S., Lammerts van Bueren, J. J., Derer, S., Lohse, S., Dechant, M.,... & Valerius, T. (2011). Complement‐mediated tumor‐specific cell lysis by antibody combinations targeting epidermal growth factor receptor (EGFR) and its variant III (EGFRvIII). Cancer science, 102(10), 1761-1768.


Specifications

  • Immunogen
  • EGFRvIII PEP3-KLH Antigen
  • Host Species
  • Human
  • Type
  • Human IgG1
  • Specificity
  • Human EGFR
  • Species Reactivity
  • Human
  • Clone
  • 13.1.2
  • Applications
  • ELISA, FC, IHC, Block, FuncS
  • Related Disease
  • Lung cancer and gliomas

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot, Flow Cytometry, Blocking and Functional Assay. Please see the Published Data for further information.

Target

  • Alternative Names
  • Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian);

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "EGFR"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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