Rabbit Anti-EGFR Recombinant Antibody (clone 1B8)
CAT#: ZG-0643J
This product is a rabbit antibody that recognizes EGFR.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-EGFR Antibody (ZG-0643J) in WB
Western Blot
Positive WB detected in A549 whole cell lysate,HepG2 whole cell lysate(treated with EGF or Pervanadate)
All lanes Phospho-EGFR antibody at 1.28μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 175 KDa
Observed band size: 175 KDa
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
Normal Tissue
Figure 7 Placenta
Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (A-431)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR
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Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-EGFR (Y1068)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human EGFR
- Species Reactivity
- Human
- Clone
- 1B8
- Applications
- ELISA, WB
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot.
WB:1:500-1:5000
Target
- Alternative Names
- ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; epidermal growth factor receptor; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor tyrosine kinase domain; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
- Gene ID
- 1956
- UniProt ID
- P00533
REVIEWS AND Q&AS
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Related Diseases
Bladder Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Hepatocellular Carcinoma
Cushing Syndrome
Related Signaling Pathways
JAK-STAT Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| NEUT-723CQ | Mouse Anti-EGFR Recombinant Antibody (clone CBL931) | WB, IP, IHC, ICC, Neut | Mouse IgG1 |
| NEUT-724CQ | Rabbit Anti-EGFR Recombinant Antibody (NEUT-724CQ) | IF, FC, WB, IP, Neut | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY84 | AbPlus™ Anti-EGFR Magnetic Beads (pSEX81-6) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-0924-YC32 | Mouse Anti-EGFR Recombinant Antibody (VS-0924-YC32) - Cancer Stem Cell Marker | IHC, WB | Mouse IgG1 |
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| VS-0125-FY28 | Human Anti-EGFR (clone ABT-806) scFv-Fc Chimera | FC, Cyt | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| VS-0225-XY102 | CytoStream™ Mouse Anti-EGFR Recombinant Antibody (VS-0225-XY102) | FC | Mouse IgG1, kappa |
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|---|---|---|---|
| VS-0325-XY735 | Anti-EGFR Immunohistochemistry Kit | IHC | |
| VS-0525-XY2183 | Anti-Mouse EGFR Immunohistochemistry Kit | IHC | |
| VS-0525-XY2182 | Anti-Human EGFR Immunohistochemistry Kit | IHC |
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