CytoStream™ Mouse Anti-EGFR Recombinant Antibody (VS-0225-XY102)

Name: CytoStream™ Mouse Anti-EGFR Recombinant Antibody (VS-0225-XY102)
SKU: VS-0225-XY102
Rating: 5 (1 reviews)

CAT#: VS-0225-XY102

Characterized by its specificity for Epidermal Growth Factor Receptor (EGFR), this antibody offers high reactivity to human samples, making it an essential tool for EGFR-related studies. With Flow Cytometry (FC) as its primary application, it enables precise cell surface analysis, contributing to the understanding of EGFR's role in cellular signaling and disease progression. Its use in various research contexts provides valuable insights into the molecular mechanisms of human health and disease.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Normal Tissue Figure 7 Placenta

Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Normal Tissue Figure 8 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (A-431)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

❮

❯

Specifications

Immunogen
Human EGF Receptor extracellular domain Recombinant Protein

Host Species
Mouse

Type
Mouse IgG1, kappa

Specificity
EGFR

Species Reactivity
Human

Applications
FC

Product Property

Purification
Protein A purified

Purity
>95%, determined by SDS-PAGE

Format
Liquid

Buffer
PBS

Preservative
Sodium azide

Storage
Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.

Target

Alternative Names
EGFR; ERBB; ERBB1; HER1; mENA; PIG61

Gene ID
1956

UniProt ID
P00533

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Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Pancreatic Cancer

Hepatocellular Carcinoma

Cushing Syndrome

Related Signaling Pathways

JAK-STAT Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0225-XY102.pdf

MSDS

MSDS - VS-0225-XY102.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Secondary Antibody

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TAB-283MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-283MZ-F(E))	ELISA	Human Fab
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NEUT-722CQ	Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011)	Neut	Rabbit IgG
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HPAB-0010-YC-F(E)	Mouse Anti-EGFR Recombinant Antibody (clone L211C); Fab Fragment	ELISA, FC	Mouse Fab
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HPAB-0012-YC-F(E)	Human Anti-EGFR Recombinant Antibody (clone Pep 2); Fab Fragment	FC	Human Fab
HPAB-0013-YC-F(E)	Human Anti-EGFR Recombinant Antibody (clone Pep 3); Fab Fragment	FC	Human Fab
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