Rabbit Anti-ELANE Recombinant Antibody (clone 2H12) (CAT#: ZG-0650J)
This product is a rabbit antibody that recognizes ELANE.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
IHC
Figure 1 Rabbit Anti-ELANE Antibody (ZG-0650J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
FC
Figure 2 Rabbit Anti-ELANE Antibody (ZG-0650J) in FC
Overlay histogram showing Jurkat cells stained with this product (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- A synthesized peptide derived from human Neutrophil Elastase
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ELANE
- Species Reactivity
- Human
- Clone
- 2H12
- Applications
- ELISA, IHC, FC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry.
IHC:1:50-1:200, FC:1:20-1:200
Target
- Alternative Names
- GE; NE; HLE; HNE; ELA2; SCN1; PMN-E; neutrophil elastase; PMN elastase; bone marrow serine protease; elastase 2, neutrophil; elastase-2; granulocyte-derived elastase; leukocyte elastase; medullasin; polymorphonuclear elastase; polymorphonuclear leukocyte elastase
- Gene ID
- 1991
- UniProt ID
- P08246
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1041 | Mouse Anti-ELANE Recombinant Antibody (clone YC039); Fab Fragment | ELISA | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
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