Rabbit Anti-EMC4 Recombinant Antibody (clone R08-6D5) (CAT#: VS3-FY1906)

This R08-6D5 recombinant monoclonal antibody is able to recognize human EMC4. It cross-reacts with mouse, rat, hamster. It is suitable for use in a variety of assays, including WB, IP. This transmembrane protein 85 antibody has been rigorously validated and is widely used in western blot analysis of TMEM85 in 3T3 lysates.

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WB

Figure 1 Anti-EMC4 Antibody (VS3-FY1906) in WB.

Figure 1 Anti-EMC4 Antibody (VS3-FY1906) in WB.

The TMEM85 expression in 3T3 lysates were detected using the anti-Transmembrane Protein 85 by western blot assay.


Specifications

  • Immunogen
  • Synthetic peptide of human TMEM85
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat, Hamster EMC4
  • Species Reactivity
  • Human, Mouse, Rat, Hamster
  • Clone
  • R08-6D5
  • Applications
  • Western Blot, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 20 kDa, while the observed molecular weight stands at 20 kDa.
  • Related Disease
  • Ogden Syndrome

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • ER Membrane Protein Complex Subunit 4; Transmembrane Protein 85; Cell Proliferation-Inducing Gene 17 Protein; TMEM85; PIG17
  • Cellular Localization
  • Endoplasmic reticulum, Membrane
  • Post Translation Modifications
  • Ubiquitination at Lys16 and Lys50 (NX_Q5J8M3 [NX_Q5J8M3-1])
    Modification sites at PhosphoSitePlus (Q5J8M3)
  • Function
  • Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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