Human Anti-ENO1 Recombinant Antibody (clone B8); Fab Fragment (CAT#: HPAB-J0074-YC-F(E))
Provided is an alpha-endolase (ENO1) -specific human antibody. The ENO1-specific human antibody may be used in diagnosis of the cancer, classification of the disease, visualization, treatment, and prognostic evaluation.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68284/1285_E3_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68284/1285_E3_4_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg
Figure 3 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/990_G8_1_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/990_G8_1_selected.jpg
Figure 4 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/ihc_selected.jpg
Figure 5 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows moderate positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows moderate positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/ihc_selected.jpg
Figure 6 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80034/170663_A_7_5_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80034/170663_A_7_5_selected.jpg
Figure 7 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/962_H4_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/962_H4_1_blue_red_green.jpg
Figure 8 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_B_8_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_B_8_5.jpg
Figure 9 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_3.jpg
Figure 10 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_4.jpg
Figure 11 Pancreas
(Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_1_3.jpg
(Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_1_3.jpg
Figure 12 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_8_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_8_5.jpg
Figure 13 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_5_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_5_6.jpg
Figure 14 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_8.jpg
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_8.jpg
Figure 15 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074800-ENO1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074800-ENO1
Specifications
- Host Species
- Human
- Type
- Human Fab
- Specificity
- Human ENO1
- Species Reactivity
- Human
- Clone
- B8
- Applications
- ELISA
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Enolase 1; 2-Phospho-D-Glycerate Hydro-Lyase; Plasminogen-Binding Protein; Phosphopyruvate Hydratase; Enolase 1, (Alpha); Non-Neural Enolase; Alpha-Enolase; EC 4.2.1.11; ENO1L1; MPB1; NNE; Epididymis Secretory Protein Li 17; C-Myc Promoter-Binding Protein-1
- Gene ID
- 2023
- UniProt ID
- P06733
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1162z | Mouse Anti-ENO1 Recombinant Antibody (clone 28F5) | WB, ELISA, IF, IHC | Mouse IgG1, κ |
| HPAB-0054-CN | Mouse Anti-ENO1 Recombinant Antibody (HPAB-0054-CN) | FC, ELISA | Mouse IgG |
| HPAB-0055-CN | Human Anti-ENO1 Recombinant Antibody (HPAB-0055-CN) | FC, ELISA | Humanized IgG |
| HPAB-0056-CN | Human Anti-ENO1 Recombinant Antibody (HPAB-0056-CN) | FC, ELISA | Humanized IgG |
| HPAB-0057-CN | Chicken Anti-ENO1 Recombinant Antibody (HPAB-0057-CN) | FC, WB, ELISA, IF | Chicken IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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