Mouse Anti-ENO1 Recombinant Antibody (clone 3F7-F3-C2)
CAT#: VS3-QX366
This product is a mouse antibody that recognizes ENO1. The antibody can be used in applications: WB, IHC.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68284/1285_E3_4_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/990_G8_1_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human kidney
Immunohistochemical staining of human kidney shows moderate positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80034/170663_A_7_5_selected.jpg
Subcellular Location and Protein Expression
Figure 7 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/962_H4_1_blue_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_B_8_5.jpg
Normal Tissue
Figure 9 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_3.jpg
Normal Tissue
Figure 10 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_4.jpg
Normal Tissue
Figure 11 Pancreas
Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_1_3.jpg
Normal Tissue
Figure 12 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_8_5.jpg
Normal Tissue
Figure 13 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_5_6.jpg
Normal Tissue
Figure 14 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_8.jpg
RNA Expression
Figure 15 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074800-ENO1
❮
❯
Specifications
- Immunogen
- Purified His-tagged ENO1 protein
- Host Species
- Mouse
- Derivation
- Mouse
- Specificity
- Enolase-1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 3F7-F3-C2
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1:1000; IHC: 1:25
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ENO1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-350MZ | Anti-Human ENO1 Recombinant Antibody (A3) | WB, FC | Human antibody |
| TAB-350MZ-S(P) | Anti-Human ENO1 Recombinant Antibody scFv Fragment (A3) | WB, FC | Human antibody |
| TAB-350MZ-F(E) | Anti-Human ENO1 Recombinant Antibody Fab Fragment (A3) | WB, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0661CT | Recombinant Mouse anti-Human ENO1 Monoclonal antibody (3H3BH22CG9) | FC, ICC, IF, IP, ELISA | |
| MRO-0535-CN | Rabbit Anti-ENO1 Recombinant Antibody (clone JM63-53) | WB, IP, FC, IF | Rabbit IgG |
| HPAB-A1275-YJ | Chicken Anti-ENO1 Recombinant Antibody (clone EnL1) | WB, ELISA | Chicken IgG |
| HPAB-A1276-YJ | Chicken Anti-ENO1 Recombinant Antibody (clone EnL4) | WB, ELISA | Chicken IgG |
| VS3-QX365 | Mouse Anti-ENO1 Recombinant Antibody (clone 3F7-B12-D10) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0190MZ | Chicken Anti-ENO1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC842 | PE-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM | |
| MHC-LC843 | APC-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM | |
| MHC-LC844 | BV421-DRB1*04:01/Human ENO1 (TSKGLFRAAVPSGAS) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1151 | Hi-Affi™ Rabbit Anti-ENO1 Recombinant Antibody (clone DS1151AB) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0054-CN-S(P) | Mouse Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0054-CN-S(P)) | FC, ELISA | Mouse scFv |
| HPAB-0055-CN-S(P) | Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0055-CN-S(P)) | FC, ELISA | Humanized scFv |
| HPAB-0056-CN-S(P) | Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0056-CN-S(P)) | FC, ELISA | Humanized scFv |
| HPAB-0057-CN-S(P) | Chicken Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0057-CN-S(P)) | FC, WB, ELISA, IF | Chicken scFv |
| HPAB-0058-CN-S(P) | Human Anti-ENO1 Recombinant Antibody; scFv Fragment (HPAB-0058-CN-S(P)) | FC, WB, ELISA, IF | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0235-CN-F(E) | Chicken Anti-ENO1 Recombinant Antibody (clone EnL2); Fab Fragment | ELISA, WB, IF, FC | Chicken Fab |
| FAMAB-0236-CN-F(E) | Chicken Anti-ENO1 Recombinant Antibody (clone EnL5); Fab Fragment | ELISA, WB, IF, FC | Chicken Fab |
| HPAB-J0072-YC-F(E) | Human Anti-ENO1 Recombinant Antibody (clone B3); Fab Fragment | ELISA | Human Fab |
| HPAB-J0073-YC-F(E) | Human Anti-ENO1 Recombinant Antibody (clone C4); Fab Fragment | ELISA | Human Fab |
| HPAB-J0074-YC-F(E) | Human Anti-ENO1 Recombinant Antibody (clone B8); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF036 | DRB1*04:01/Human cit alpha-enolase (IFDS[cit]GNPTVEVDLF) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1382 | AbPlus™ Anti-Eno1 Magnetic Beads (VS-0724-YC1382) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY767 | Anti-ENO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2287 | Anti-Mouse ENO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2286 | Anti-Human ENO1 Immunohistochemistry Kit | IHC |
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