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    Rabbit Anti-ENO1 Recombinant Antibody (clone JM63-53) (CAT#: MRO-0535-CN)

    This antibody is a rabbit monoclonal antibody that is capable of binding to ENO1.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68284/1285_E3_4_selected.jpg
    Figure 2 IF staining of human cell line A-431
    Figure 2 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg
    Figure 3 IF staining of human cell line A549
    Figure 3 IF staining of human cell line A549
    (Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/990_G8_1_selected.jpg
    Figure 4 IHC staining of human pancreas
    Figure 4 IHC staining of human pancreas
    (Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/ihc_selected.jpg
    Figure 5 IHC staining of human kidney
    Figure 5 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows moderate positivity.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/ihc_selected.jpg
    Figure 6 IHC staining of human kidney
    Figure 6 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80034/170663_A_7_5_selected.jpg
    Figure 7 IF staining of human cell line MCF7
    Figure 7 IF staining of human cell line MCF7
    (Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/962_H4_1_blue_red_green.jpg
    Normal Tissue
    Figure 8 Cerebral cortex
    Figure 8 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Neuronal cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear
    Neuropil
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_B_8_5.jpg
    Figure 9 Colon
    Figure 9 Colon
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Glandular cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear
    Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_3.jpg
    Figure 10 Liver
    Figure 10 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear
    Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_4.jpg
    Figure 11 Pancreas
    Figure 11 Pancreas
    (Exocrine glandular cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_1_3.jpg
    Figure 12 Kidney
    Figure 12 Kidney
    (Cells in glomeruli
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear
    Cells in tubules
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_8_5.jpg
    Figure 13 Testis
    Figure 13 Testis
    (Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_5_6.jpg
    Figure 14 Lymph node
    Figure 14 Lymph node
    (Germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Non-germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_8.jpg
    RNA Expression
    Figure 15 RNA cell line category: Low cell line specificity
    Figure 15 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074800-ENO1

    Specifications

    • Immunogen
    • Synthetic peptide within N-terminal of human ENO1
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human ENO1
    • Species Reactivity
    • Human, Mouse, Rat, Zebrafish
    • Clone
    • JM63-53
    • Applications
    • WB, IP, FC, IF
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Purified with Protein A affinity chromatography
    • Purity
    • >95% as determined by SDS-PAGE
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • TBS, pH 7.4, 1% BSA, 40% Glycerol
    • Preservative
    • 0.05% Sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Target

    • Alternative Names
    • Enolase 1; 2-Phospho-D-Glycerate Hydro-Lyase; Plasminogen-Binding Protein; Phosphopyruvate Hydratase; Enolase 1, (Alpha); Non-Neural Enolase; Alpha-Enolase; EC 4.2.1.11; ENO1L1; MPB1; NNE; Epididymis Secretory Protein Li 17; C-Myc Promoter-Binding Protein-1;

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    CAT Product Name Application Type
    TAB-350MZ Anti-Human ENO1 Recombinant Antibody (A3) WB, FC Human antibody
    TAB-350MZ-S(P) Anti-Human ENO1 Recombinant Antibody scFv Fragment (A3) WB, FC Human antibody
    TAB-350MZ-F(E) Anti-Human ENO1 Recombinant Antibody Fab Fragment (A3) WB, FC Human antibody

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