Rabbit Anti-ENPP5 Recombinant Antibody (clone R95-5O-8) (CAT#: VS3-FY2175)
This R95-5O-8 recombinant monoclonal antibody is able to recognize human ENPP5 protein, a calculated weight of 55 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R95-5O-8 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunofluorescence (dilution range 1:50-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RPTEC TERT1
(Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16902/1789_A5_9_cr597063c33efc9_selected.jpg
(Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16902/1789_A5_9_cr597063c33efc9_selected.jpg
Figure 2 High expression in epididymis
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16902/38911_A_2_6_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16902/38911_A_2_6_val_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16902/38911_B_9_5.jpg
(Neuronal cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16902/38911_B_9_5.jpg
Figure 4 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16902/38911_A_9_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16902/38911_A_9_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16902/38911_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16902/38911_A_8_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16902/38911_A_6_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16902/38911_A_6_6.jpg
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, HSkMC, SuSa, T-47d, U-266/84)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112796-ENPP5
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112796-ENPP5
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ENPP5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R95-5O-8
- Applications
- Western Blot, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 55kDa, while the observed molecular weight stands at 55kDa.
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Ectonucleotide Pyrophosphatase/Phosphodiesterase 5 (Putative); E-NPP 5; NPP-5; Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 5; EC 3.1.-.-; NPP5
- Gene ID
- 59084
- UniProt ID
- Q9UJA9
- Sequence Similarities
- Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
- Cellular Localization
- Membrane, Secreted
- Post Translation Modifications
- N-glycosylated. (Q9UJA9-ENPP5_HUMAN)
Glycosylation at Asn101, Asn158, Asn292, Asn329, Asn362, Asn369, Asn382, and Asn389 (NX_Q9UJA9 [NX_Q9UJA9-1])
Modification sites at PhosphoSitePlus (Q9UJA9)
Glycosylation from GlyGen (Q9UJA9) 9 sites, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005249317.1; XP_011513088.1; XP_054212113.1; XP_054212114.1; NP_001277001.1
- Function
- Can hydrolyze NAD but cannot hydrolyze nucleotide di- and triphosphates. Lacks lysopholipase D activity. May play a role in neuronal cell communication.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
Rabbit Monoclonal Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0666CT | Recombinant Mouse anti-Human ENPP5 Monoclonal antibody (EML1696) | WB |
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