Mouse Anti-Enrofloxacin Recombinant Antibody (clone Enro44) (CAT#: FAMAB-0126CQ)
Recombinant Mouse Antibody clone Enro44, which is specific to Enrofloxacin. The antibody, Enro44, which can be used in a direct competitive ELISA to detect enrofloxacin with high specificity, sensitivity, and precision. It is sufficient for screening enrofloxacin in samples including chicken muscle, cattle milk, and urine within the range of the enforced MRL.
Specific Inquiry
ELISA
Figure 1 Dose-response curve of the monoclonal antibody Enro44 analyzed by Ag-captured direct competitive ELISA.
B0 and B are the average absorbances obtained from the ELISA without and with different concentrations of the competitor, respectively.
Chusri, M., Wongphanit, P., Palaga, T., Puthong, S., Sooksai, S., & Komolpis, K. (2013). Production and characterization of a monoclonal antibody against enrofloxacin. J Microbiol Biotechnol, 23(1), 69-75.
Specifications
- Immunogen
- Enrofloxacin-BSA conjugate
- Host Species
- Mouse
- Type
- Mouse IgG1, λ
- Clone
- Enro44
- Applications
- ELISA
- Related Disease
- Infectious diseases in the livestock industry
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Enzyme-linked Immunosorbent Assay
For hybridoma screening, MaxiSorp microtiter plates (Nunc, Roskilde, Denmark) were coated with 3 µg/ml of enrofloxacin-BSA in 0.1 M phosphate-buffered saline (PBS), pH 7.4, at 4°C, overnight. Plates were washed with PBS and blocked with 0.2% skim milk powder in PBS at 37°C for 1 h. After washing, the culture supernatant or sera were added, and plates were incubated at 37°C for 2 h. After another washing step, horseradish peroxidase (HRP)-conjugated anti-mouse rabbit antibody (Zymed, CA, USA) at a 1:2,000 dilution in 0.5% BSA was added to each well and incubated at 37°C for 1 h. The unbound antibody was removed by washing with PBS. Peroxidase substrate [150 µl of 0.01 M o-phenylendiamine dihydrochloride in 0.1 M citrate-phosphate buffer, pH 5.0, containing 0.001% (v/v) H₂O₂] was added. The reaction was allowed to proceed for 15 min at room temperature in the dark. The enzymatic reaction was terminated with 100 µl of 2.5 M H₂SO₄, and the absorbance was measured at 492 nm with a microtiter plate reader (Titertek Multiskan, CA, USA). For isotype determination, antibody isotyping kits (Sigma-Aldrich) were used.
Target
- Alternative Names
- Enrofloxacin; Baytril
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-320 | Recombinant Anti-Enrofloxacin Antibody | ELISA, IP, FuncS | IgG |
| MHH-320 | Recombinant Human Anti-Enrofloxacin Antibody | IF, FACS, FuncS | IgG |
| FAMAB-0127CQ | Mouse Anti-Enrofloxacin Recombinant Antibody (clone VHenro48-2) | ELISA | Mouse IgG |
| FAMAB-0128CQ | Mouse Anti-Enrofloxacin Recombinant Antibody (clone VHnor155-9) | ELISA | Mouse IgG |
| MRO-A1850-YJ | Mouse Anti-Enrofloxacin Recombinant Antibody (clone CBS371) | ELISA | Mouse IgM |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-320-F(E) | Recombinant Anti-Enrofloxacin Antibody Fab Fragment | WB, ELISA, FuncS | Fab |
| MHH-320-F(E) | Recombinant Human Anti-Enrofloxacin Antibody Fab Fragment | WB, Neut, FuncS | Fab |
| FAMAB-0126CQ-F(E) | Mouse Anti-Enrofloxacin Recombinant Antibody (clone Enro44); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0127CQ-F(E) | Mouse Anti-Enrofloxacin Recombinant Antibody (clone VHenro48-2); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0128CQ-F(E) | Mouse Anti-Enrofloxacin Recombinant Antibody (clone VHnor155-9); Fab Fragment | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-320-S(P) | Recombinant Anti-Enrofloxacin Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| MHH-320-S(P) | Recombinant Human Anti-Enrofloxacin Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| FAMAB-0126CQ-S(P) | Mouse Anti-Enrofloxacin Recombinant Antibody (clone Enro44); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0127CQ-S(P) | Mouse Anti-Enrofloxacin Recombinant Antibody (clone VHenro48-2); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0128CQ-S(P) | Mouse Anti-Enrofloxacin Recombinant Antibody (clone VHnor155-9); scFv Fragment | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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