Mouse Anti-Enrofloxacin Recombinant Antibody (clone Enro44)
CAT#: FAMAB-0126CQ
Recombinant Mouse Antibody clone Enro44, which is specific to Enrofloxacin. The antibody, Enro44, which can be used in a direct competitive ELISA to detect enrofloxacin with high specificity, sensitivity, and precision. It is sufficient for screening enrofloxacin in samples including chicken muscle, cattle milk, and urine within the range of the enforced MRL.
Specifications
- Immunogen
- Enrofloxacin-BSA conjugate
- Host Species
- Mouse
- Type
- Mouse IgG1, λ
- Clone
- Enro44
- Applications
- ELISA
- Related Disease
- Infectious diseases in the livestock industry
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Enzyme-linked Immunosorbent Assay
For hybridoma screening, MaxiSorp microtiter plates (Nunc, Roskilde, Denmark) were coated with 3 µg/ml of enrofloxacin-BSA in 0.1 M phosphate-buffered saline (PBS), pH 7.4, at 4°C, overnight. Plates were washed with PBS and blocked with 0.2% skim milk powder in PBS at 37°C for 1 h. After washing, the culture supernatant or sera were added, and plates were incubated at 37°C for 2 h. After another washing step, horseradish peroxidase (HRP)-conjugated anti-mouse rabbit antibody (Zymed, CA, USA) at a 1:2,000 dilution in 0.5% BSA was added to each well and incubated at 37°C for 1 h. The unbound antibody was removed by washing with PBS. Peroxidase substrate [150 µl of 0.01 M o-phenylendiamine dihydrochloride in 0.1 M citrate-phosphate buffer, pH 5.0, containing 0.001% (v/v) H₂O₂] was added. The reaction was allowed to proceed for 15 min at room temperature in the dark. The enzymatic reaction was terminated with 100 µl of 2.5 M H₂SO₄, and the absorbance was measured at 492 nm with a microtiter plate reader (Titertek Multiskan, CA, USA). For isotype determination, antibody isotyping kits (Sigma-Aldrich) were used.
Target
- Alternative Names
- Enrofloxacin; Baytril
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone Enro44"
- CAT
- Product Name
See other products for "Enrofloxacin"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-320 | Recombinant Anti-Enrofloxacin Antibody | ELISA, IP, FuncS | IgG |
| MHH-320 | Recombinant Human Anti-Enrofloxacin Antibody | IF, FACS, FuncS | IgG |
| FAMAB-0127CQ | Mouse Anti-Enrofloxacin Recombinant Antibody (clone VHenro48-2) | ELISA | Mouse IgG |
| FAMAB-0128CQ | Mouse Anti-Enrofloxacin Recombinant Antibody (clone VHnor155-9) | ELISA | Mouse IgG |
| ZG-0213U | Mouse Anti-Enrofloxacin Recombinant Antibody (clone 7D1F1) | ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-320-F(E) | Recombinant Anti-Enrofloxacin Antibody Fab Fragment | WB, ELISA, FuncS | Fab |
| MHH-320-F(E) | Recombinant Human Anti-Enrofloxacin Antibody Fab Fragment | WB, Neut, FuncS | Fab |
| FAMAB-0126CQ-F(E) | Mouse Anti-Enrofloxacin Recombinant Antibody (clone Enro44); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0127CQ-F(E) | Mouse Anti-Enrofloxacin Recombinant Antibody (clone VHenro48-2); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0128CQ-F(E) | Mouse Anti-Enrofloxacin Recombinant Antibody (clone VHnor155-9); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-320-S(P) | Recombinant Anti-Enrofloxacin Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| MHH-320-S(P) | Recombinant Human Anti-Enrofloxacin Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| FAMAB-0126CQ-S(P) | Mouse Anti-Enrofloxacin Recombinant Antibody (clone Enro44); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0127CQ-S(P) | Mouse Anti-Enrofloxacin Recombinant Antibody (clone VHenro48-2); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0128CQ-S(P) | Mouse Anti-Enrofloxacin Recombinant Antibody (clone VHnor155-9); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-A1850-YJ | Mouse Anti-Enrofloxacin Recombinant Antibody (clone CBS371) | ELISA | Mouse IgM |
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