Mouse Anti-EPAS1 Recombinant Antibody (clone 1H3E12) (CAT#: VS7-0425-WR35)
This product is a mouse IgG1 recombinant antibody that binds to human EPAS1. The antibody 1H3E12 recognizes a specific amino acid domains: 680-870. It can be used for IHC, ICC, adnd FC assays.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31200/1391_B7_1_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31200/1391_B7_1_selected.jpg
Figure 2 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & actin filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69697/1382_A10_1_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & actin filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69697/1382_A10_1_selected.jpg
Figure 3 RNA cell line category: Cell line enhanced (BEWO, BJ hTERT+, HHSteC, RT4, TIME, U-138 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116016-EPAS1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116016-EPAS1
Specifications
- Immunogen
- Recombinant human EPAS1 (AA: 680-870) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human EPAS1
- Species Reactivity
- Human
- Clone
- 1H3E12
- Molecule Class
- Transcription Factor
- Applications
- IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Familial erythrocytosis; Pheochromocytoma/paraganglioma; Congenital heart disease; Lynch syndrome
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
- Gene ID
- 2034
- UniProt ID
- Q99814
- Long Name
- Endothelial PAS domain protein 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Hydroxylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001421
- Function
- Activator; Developmental protein; DNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "EPAS1"
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4055z | Mouse Anti-EPAS1 Recombinant Antibody (clone 13F1) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-0918CT | Recombinant Mouse anti-Human EPAS1 Monoclonal antibody (fq200c) | ELISA, IHC-Fr, IHC-P, WB | |
| MRO-0737-CN | Rabbit Anti-EPAS1 Recombinant Antibody (clone JB24-42) | WB, IHC, FC | Rabbit IgG |
| ZG-0651J | Rabbit Anti-EPAS1 Recombinant Antibody (clone 6A9) | ELISA, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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