Mouse Anti-EPCAM Recombinant Antibody (clone 2A9) (CAT#: HPAB-1520-FY)

Figure 1 Binding ability of 2A9 and 2D7 to EpCAM was detected on Colo205 and HHCC cells by flow cytometry.

Cells were incubated with biotin labeled EpCAM antibody (2A9 or 2D7, red histograms) or isotype control (black histograms) antibody (20 μg/mL) for 40 min at 4°C. Then cells were washed and stained with FITC-labeled secondary antibody and analyzed by flow cytometry.

Lv, M., Qiu, F., Li, T., Sun, Y., Zhang, C., Zhu, P., ... & Zhang, K. (2015). Construction, expression, and characterization of a recombinant immunotoxin targeting EpCAM. Mediators of inflammation, 2015.

Figure 2 Binding ability of immunotoxin to recombinant His-EpCAM and natural EpCAM.

HHCC cells were collected and stained with primary antibody (biotin labeled immunotoxin, biotin labeled 2A9, or biotin labeled isotype control antibody) at a concentration of 5 μg/mL and FITC labeled avidin (1 : 50) as the secondary antibody. Fluorescence was detected by BD FACS Calibur. The mean fluorescent intensity was measured by FlowJo software.

Lv, M., Qiu, F., Li, T., Sun, Y., Zhang, C., Zhu, P., ... & Zhang, K. (2015). Construction, expression, and characterization of a recombinant immunotoxin targeting EpCAM. Mediators of inflammation, 2015.