Human Anti-EPCAM Recombinant Antibody (clone D4.5)

Name: Human Anti-EPCAM Recombinant Antibody (clone D4.5)
SKU: HPAB-A1307-YJ
Rating: 5 (1 reviews)

CAT#: HPAB-A1307-YJ

This product is a chimeric (mouse/human) antibody that recognizes EPCAM.

Gene Expression Published Data

Subcellular Location Figure 1 IF staining of human cell line MCF7

Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67463/1592_H11_2_selected.jpg

Normal Tissue Figure 2 Thyroid gland

Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_B_2_5.jpg

Normal Tissue Figure 3 Colon

Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_9_3.jpg

Normal Tissue Figure 4 Kidney

Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_8_5.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (A-431, CACO-2, CAPAN-2, OE19, RPTEC TERT1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM

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ELISA

Figure 1 ELISA of soluble Fab fragments.

Figure 2 Flow cytometry analysis of Fab antibody fragments and complete antibodies on 17-1A positive Kato cells.

Irrelevant pp (broken line) or kS.1-D4.5-pp (solid line).

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Specifications

Host Species
Human

Derivation
Chimeric (mouse/human)

Type
Chimeric (mouse/human) IgG1

Specificity
Human EPCAM

Species Reactivity
Human

Clone
D4.5

Applications
Enzyme-linked Immunosorbent Assay and Flow Cytometry

Product Property

Purification
Protein A/G Purified

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.

Buffer
PBS

Storage
Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Enzyme-linked Immunosorbent Assay and Flow Cytometry. Optimal working dilutions must be determined by the end user.

Target

Alternative Names
ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; 17-A1; EGP; GA 733-2; epithelial cell adhesion molecule; adenocarcinoma-associated antigen; cell surface glycoprotein Trop-1; epithelial glycoprotein 314; human epithelial glycoprotein-2; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); trophoblast cell surface antigen 1; tumor-associated calcium signal transducer 1

Gene ID
4072

UniProt ID
P16422

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-A1307-YJ.pdf

MSDS

MSDS - HPAB-A1307-YJ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

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