Human Anti-EpCAM Recombinant Antibody (clone K53) (CAT#: HPAB-2050-FY)

This product is a recombinant human antibody that recognizes EpCAM. The monoclonal human antibody K53 reacts with the epitope of EpCAM.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
SPR

Figure 1 BIACORE analysis of K53.

Figure 1 BIACORE analysis of K53.

Affinity purified human CD46 from LS174T cells was coupled (640 RU) to CM5 chips (BIACORE BR-1000-14) Binding of 1000 (A), 500 (B) , 250 (C), 125 (D) , 63 (E) , 31 (F) , 16 (G) , 8 (H) or 0 (I) nM 02-237 or k53 purified from stable PER.C6TM derived cell lines to the CD46 was monitored using a BIACORE 3000 system at 37°C.

FC

Figure 2 Binding of K53 and 02-237 to LS174T cells.

Figure 2 Binding of K53 and 02-237 to LS174T cells.

PP

Figure 3 SDS-PAGE analysis of purified IgG fractions.

Figure 3 SDS-PAGE analysis of purified IgG fractions.

Three μg purified IgG was analysed on a reduced 4-20% Nupage gel (Novex) according to recommendations of the manufacturer.

FC

Figure 4 FACS analysis of mixtures of antibodies produced from sub-clones of poly 1-241.

Figure 4 FACS analysis of mixtures of antibodies produced from sub-clones of poly 1-241.

Binding of the mixtures of antibodies to cells transfected with cDNA of CD46, EpCAM, or a negative control (CD38), was determined with FACS analysis, and mean fluorescent intensity (MFI) is shown for the various parent clones and three independent sub-clones of each.


Specifications

  • Host Species
  • Human
  • Type
  • Human IgG1, κ
  • Specificity
  • Human EpCAM
  • Species Reactivity
  • Human
  • Clone
  • K53
  • Applications
  • SPR, FC, PP

Product Property

  • Purity
  • >95% as determined by SDS-PAGE analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The EpCAM antibody has been reported in applications of Surface Plasmon Resonance, Flow Cytometry, Protein Purification.

Target

  • Alternative Names
  • Epithelial Cell Adhesion Molecule; Tumor-Associated Calcium Signal Transducer 1; Major Gastrointestinal Tumor-Associated Protein GA733-2; Adenocarcinoma-Associated Antigen; Cell Surface Glycoprotein Trop-1; Epithelial Glycoprotein 314; TACSTD1; EGP314; MIC18; TROP1; M4S1; KSA; Membrane Component, Chromosome 4, Surface Marker (35kD Glycoprotein); Antigen Identified By Monoclonal Antibody AUA1; Human Epithelial Glycoprotein-2; Epithelial Cell Surface Antigen

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone K53"

See other products for "EPCAM"

Mouse Antibody

Humanized Antibody

Human Antibody

Immunotoxin

CAT Product Name Application Type
AGTO-L013E anti-EPCAM immunotoxin MOC31 (IgG)-PE Cytotoxicity assay, Functional assay
AGTO-L013D anti-EPCAM immunotoxin MOC31 (IgG)-DT Cytotoxicity assay, Functional assay
AGTO-L013G anti-EPCAM immunotoxin MOC31 (IgG)-Gel Cytotoxicity assay, Functional assay
AGTO-L013R anti-EPCAM immunotoxin MOC31 (IgG)-RTA Cytotoxicity assay, Functional assay
AGTO-L013O anti-EPCAM immunotoxin MOC31 (IgG)-Bouganin Cytotoxicity assay, Functional assay

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1163 Hi-Affi™ Rabbit Anti-EPCAM Recombinant Antibody (clone DS1163AB) IHC-P, WB, ICC, ICC, IF Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-106 Afuco™ Anti-EPCAM ADCC Recombinant Antibody (Adecatumumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut ADCC enhanced antibody
AFC-TAB-213 Afuco™ Anti-EPCAM ADCC Recombinant Antibody (Tucotuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS ADCC enhanced antibody
AFC-TAB-701 Afuco™ Anti-EpCAM ADCC Recombinant Antibody (Edrecolomab), ADCC Enhanced ELISA, IP, FC, FuncS, IF, Neut ADCC enhanced antibody

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for HPAB-2050-FY. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare