Rabbit Anti-EPCAM Recombinant Antibody (VS3-CJ701)

Name: Rabbit Anti-EPCAM Recombinant Antibody (VS3-CJ701)
SKU: VS3-CJ701
Rating: 5 (1 reviews)

CAT#: VS3-CJ701

This product is a rabbit antibody that recognizes human, mouse, and rat EPCAM (CD326).

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line MCF7

Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67463/1592_H11_2_selected.jpg

Normal Tissue Figure 2 Thyroid gland

Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_B_2_5.jpg

Normal Tissue Figure 3 Colon

Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_9_3.jpg

Normal Tissue Figure 4 Kidney

Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_8_5.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (A-431, CACO-2, CAPAN-2, OE19, RPTEC TERT1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM

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Specifications

Immunogen
Recombinant protein

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat EPCAM (CD326)

Species Reactivity
Human, Mouse, Rat

Applications
WB, IHC

Conjugate
Unconjugated

Product Property

Purification
Protein A affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Buffer
40% Glycerol, 1% BSA, TBS, pH7.4.

Preservative
0.05% Sodium Azide

Storage
Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Western Blot, Immunohistochemistry.

Target

Alternative Names
ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; BerEp4; EGP314; HNPCC8; MOC-31; Ber-Ep4; TACSTD1

Gene ID
4072

UniProt ID
P16422

Sequence Similarities
Belongs to the EPCAM family.

Cellular Localization
Cell junction, Cell membrane, Membrane, Tight junction

Post Translation Modifications
Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.

Protein Refseq
NP_002345.2

Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-CJ701.pdf

MSDS

MSDS - VS3-CJ701.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

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