Mouse Anti-EPHA5 Recombinant Antibody (clone 11C12)
CAT#: HPAB-0010-WJ
This product is a mouse monoclonal antibody that recognizes human EphA5. The antibody clone reacts with the the extracellular region (aa 304-467) of target. The binding of this antibody is specific for human EphA5 protein.
Published Data
Gene Expression
ELISA
Figure 1 Validation of anti-EphA5 monoclonal antibody 11C12.
A, ELISA showing binding of 11C12 to human and rat EphA5. The monoclonal antibody 11C12 does not recognize mouse EphA5.
Staquicini, F. I., Qian, M. D., Salameh, A., Dobroff, A. S., Edwards, J. K., Cimino, D. F., ... & Liu, D. D. (2015). Receptor tyrosine kinase EphA5 is a functional molecular target in human lung cancer. Journal of Biological Chemistry, 290(12), 7345-7359.
IF
Figure 2 Validation of anti-EphA5 monoclonal antibody 11C12.
D and E, receptor-mediated internalization (D) and receptor degradation (E) were also evaluated.
Staquicini, F. I., Qian, M. D., Salameh, A., Dobroff, A. S., Edwards, J. K., Cimino, D. F., ... & Liu, D. D. (2015). Receptor tyrosine kinase EphA5 is a functional molecular target in human lung cancer. Journal of Biological Chemistry, 290(12), 7345-7359.
IB
Figure 3 Validation of anti-EphA5 monoclonal antibody 11C12.
D and E, receptor-mediated internalization (D) and receptor degradation (E) were also evaluated.
Staquicini, F. I., Qian, M. D., Salameh, A., Dobroff, A. S., Edwards, J. K., Cimino, D. F., ... & Liu, D. D. (2015). Receptor tyrosine kinase EphA5 is a functional molecular target in human lung cancer. Journal of Biological Chemistry, 290(12), 7345-7359.
Inhib
Figure 4 The monoclonal antibody 11C12 radiosensitizes lung cancer in vivo.
A, growth inhibitory effect of the combined treatment of mAb 11C12 plus radiotherapy in vivo. Cohorts consisted of at least eight mice; the data are reported as mean tumor volumes ±S.D.
Staquicini, F. I., Qian, M. D., Salameh, A., Dobroff, A. S., Edwards, J. K., Cimino, D. F., ... & Liu, D. D. (2015). Receptor tyrosine kinase EphA5 is a functional molecular target in human lung cancer. Journal of Biological Chemistry, 290(12), 7345-7359.
IHC
Figure 5 The monoclonal antibody 11C12 radiosensitizes lung cancer in vivo.
C andD, quantification of Ki67-positive cells in tumor specimens collected 48 h after the end of each treatment. Photomicrographs illustrate Ki67-positive tumor cells.
Staquicini, F. I., Qian, M. D., Salameh, A., Dobroff, A. S., Edwards, J. K., Cimino, D. F., ... & Liu, D. D. (2015). Receptor tyrosine kinase EphA5 is a functional molecular target in human lung cancer. Journal of Biological Chemistry, 290(12), 7345-7359.
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RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (hTERT-RPE1, PC-3, TIME, U-138 MG, U-2197, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000145242-EPHA5
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Specifications
- Immunogen
- EphA polypeptide
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human EPHA5
- Species Reactivity
- Human
- Clone
- 11C12
- Applications
- ELISA
- Related Disease
- Lung cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The EPHA5 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunofluorescence, Immunoblotting, Inhibition, Immunohistochemistry.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0653CT | Recombinant Mouse anti-Human EPHA5 Monoclonal antibody (9C20C2,9C20G6) | ELISA, WB | |
| ZG-0296J | Mouse Anti-EPHA5 Recombinant Antibody (ZG-0296J) | WB, ELISA | Mouse IgG |
| VS3-QX372 | Mouse Anti-EPHA5 Recombinant Antibody (clone 46CT61.6.4) | WB, IHC | Mouse IgG1, κ |
| VS7-HM873 | Mouse Anti-EPHA5 Recombinant Antibody (clone CBL236HM) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0010-WJ-S(P) | Mouse Anti-EPHA5 Recombinant Antibody (clone 11C12); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0010-WJ-F(E) | Mouse Anti-EPHA5 Recombinant Antibody (clone 11C12); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY178 | Human Anti-EphA5 (clone 11C12) scFv-Fc Chimera | ELISA, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC679 | Recombinant Anti-EPHA5 Vesicular Antibody, EV Displayed (VS-0425-YC679) | ELISA, FC, Cell-uptake |
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