Mouse Anti-EPHB2 Recombinant Antibody (ZG-0301J)
CAT#: ZG-0301J
This product is a mouse antibody that recognizes EPHB2.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-EPHB2 Recombinant Antibody (ZG-0301J) in WB
Western Blot analysis using EphB2 Monoclonal Antibody against truncated EphB2 recombinant protein (1) and extracellular EphB2(aa19-476)-hIgGFc transfected CHO-K1 cell lysate(2)
IF
Figure 2 Mouse Anti-EPHB2 Recombinant Antibody (ZG-0301J) in IF
Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using EphB2 Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71200/1400_A5_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (GAMG, HUVEC TERT2, TIME, U-251 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000133216-EPHB2
❮
❯
Specifications
- Immunogen
- Purified recombinant fragment of EphB2 (aa17-200) expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human EPHB2
- Species Reactivity
- Human
- Applications
- WB, IF, ELISA
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunofluorescence, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5; BDPLT22; ephrin type-B receptor 2; EPH-like kinase 5; developmentally-regulated Eph-related tyrosine kinase; elk-related tyrosine kinase; eph tyrosine kinase 3; protein-tyrosine kinase HEK5; renal carcinoma antigen NY-REN-47; tyrosine-protein kinase TYRO5; tyrosine-protein kinase receptor EPH-3
- Gene ID
- 2048
- UniProt ID
- B4DSE0
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0301J. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "EPHB2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0657CT | Recombinant Mouse anti-Human EPHB2 Monoclonal antibody (EML072) | WB | |
| HPAB-0292-CN | Mouse Anti-EPHB2 Recombinant Antibody (HPAB-0292-CN) | ELISA, WB, FC, IHC, IF, IP | Mouse IgG1 |
| VS3-QX375 | Mouse Anti-EPHB2 Recombinant Antibody (clone 48CT12.6.4) | WB, IHC, FC | Mouse IgG1 |
| VS3-XY634 | Mouse Anti-EPHB2 Recombinant Antibody (clone 2D12C6) | ELISA, WB, ICC | Mouse IgG2b |
| VS7-HM878 | Mouse Anti-EPHB2 Recombinant Antibody (clone CBL241HM) | WB, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0292-CN-S(P) | Mouse Anti-EPHB2 Recombinant Antibody; scFv Fragment (HPAB-0292-CN-S(P)) | ELISA, WB, FC, IHC, IF, IP | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0292-CN-F(E) | Mouse Anti-EPHB2 Recombinant Antibody; Fab Fragment (HPAB-0292-CN-F(E)) | ELISA, WB, FC, IHC, IF, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY36 | Mouse Anti-EPHB2 (clone 2H9) scFv-Fc Chimera | ELISA, IHC, FC, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC585 | Recombinant Anti-EPHB2 Vesicular Antibody, EV Displayed (VS-0425-YC585) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2315 | Anti-Mouse EPHB2 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: Block, Cyt, FuncS, Inhib
Application: WB, IF, FuncS
Application: ELISA, FC, Inhib
Application: ELISA, FC, Inhib, IHC-Fr, WB, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
