Mouse Anti-ERBB3 Recombinant Antibody (clone 09D03) (CAT#: HPAB-M0348-YC)

Provided is a monoclonal antibody combing and inhibiting activation of epidermal growth factor relevant members Erbb3/Her3. The KD value of 09D03 to ERBB3 is 55 pM. The antibody can be used for the esophagus cancer research.


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  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA

Figure 1A-1B are graphs summarizing results from an experiment to measure the neutralization activity of negative control (murine IgG (Δ)) and anti-ErbB3 monoclonal antibodies 04D01 (▪), 12A07 (◯), 18H02 (▯), 22A02 (□) and 24C05 (Q) to inhibit NRG1-β1 binding to hErbB3 (Figure 1A) and to measure the enhanced binding of NRG1-β1 to rhErbB3 by the anti-ErbB3 mAb 09D03 (▴) and 11G01 (*) (Figure 1B).

Figure 1A-1B are graphs summarizing results from an experiment to measure the neutralization activity of negative control (murine IgG (Δ)) and anti-ErbB3 monoclonal antibodies 04D01 (▪), 12A07 (◯), 18H02 (▯), 22A02 (□) and 24C05 (Q) to inhibit NRG1-β1 binding to hErbB3 (Figure 1A) and to measure the enhanced binding of NRG1-β1 to rhErbB3 by the anti-ErbB3 mAb 09D03 (▴) and 11G01 (*) (Figure 1B).

Figure 2 is a graph summarizing results from an experiment to measure the neutralization activity of negative control (murine IgG) and anti-ErbB3 monoclonal antibodies 04D01, 09D03, 11G01, 12A07, 18H02, 22A02 and 24C05 to inhibit NRG1-al binding to rhErbB3.

Figure 2 is a graph summarizing results from an experiment to measure the neutralization activity of negative control (murine IgG) and anti-ErbB3 monoclonal antibodies 04D01, 09D03, 11G01, 12A07, 18H02, 22A02 and 24C05 to inhibit NRG1-al binding to rhErbB3.

Figure 3 is a graph summarizing results from an experiment to measure the cell surface recognition of the anti-ErbB3 antibodies of the chimeric protein Her2/3d2 expressed at the surface of CHO cells.

Figure 3 is a graph summarizing results from an experiment to measure the cell surface recognition of the anti-ErbB3 antibodies of the chimeric protein Her2/3d2 expressed at the surface of CHO cells.

Figure 4 is a graph summarizing results from an experiment to measure the anti-proliferation activity of negative control IgG (murine IgG (Δ)) and anti-ErbB3 monoclonal antibodies 04D01 (▪), 09D03 (▾), 11G01 (♦), 12A07 (◯), 18H02 (⋄), 22A02 (▯) and 24C05 (□) in BaF/3 cells expressing Her2 and ErbB3 in presence of NRG1-β1.

Figure 4 is a graph summarizing results from an experiment to measure the anti-proliferation activity of negative control IgG (murine IgG (Δ)) and anti-ErbB3 monoclonal antibodies 04D01 (▪), 09D03 (▾), 11G01 (♦), 12A07 (◯), 18H02 (⋄), 22A02 (▯) and 24C05 (□) in BaF/3 cells expressing Her2 and ErbB3 in presence of NRG1-β1.

Figure 5 is a graph summarizing results from an experiment to measure the anti-proliferation activity of anti-ErbB3 monoclonal antibodies 04D01 (▪), 09D03 (▾), 11G01 (♦), 12A07 (▪), 18H02 (⋄), 22A02 (▯) and 24C05 (□) in MCF7 cells in the presence of NRG1-β1.

Figure 5 is a graph summarizing results from an experiment to measure the anti-proliferation activity of anti-ErbB3 monoclonal antibodies 04D01 (▪), 09D03 (▾), 11G01 (♦), 12A07 (▪), 18H02 (⋄), 22A02 (▯) and 24C05 (□) in MCF7 cells in the presence of NRG1-β1.

Figure 6 is a graph summarizing results from an experiment to measure the anti-proliferation activity of negative control (murine IgG) and anti-ErbB3 monoclonal antibodies 04D01, 09D03, 11G01, 12A07, 18H02, 22A02 and 24C05 in SKBR-3 cells treated with 5 μg/ml of antibodies in the presence of serum.

Figure 6 is a graph summarizing results from an experiment to measure the anti-proliferation activity of negative control (murine IgG) and anti-ErbB3 monoclonal antibodies 04D01, 09D03, 11G01, 12A07, 18H02, 22A02 and 24C05 in SKBR-3 cells treated with 5 μg/ml of antibodies in the presence of serum.

Figure 7 is graph summarizing results from an experiment to measure the inhibitory activity of negative control IgG and anti-ErbB3 monoclonal antibodies 04D01, 09D03, 11G01, 12A07, 18H02, 22A02 and 24C05 on the phosphorylation of ErbB3 induced by NRG in SKBR-3 cells.

Figure 7 is graph summarizing results from an experiment to measure the inhibitory activity of negative control IgG and anti-ErbB3 monoclonal antibodies 04D01, 09D03, 11G01, 12A07, 18H02, 22A02 and 24C05 on the phosphorylation of ErbB3 induced by NRG in SKBR-3 cells.

FuncS

Figure 8 is a graph summarizing results from an experiment to measure the tumor inhibitory activity of the anti-ErbB3 antibodies 04D01 (Δ), 09D03 (*), 11G01 (□), 12A07 (▴), 18H02 (▯), 22A02 (▪), 24C05 (◯) and a human IgG control (- -▪- -) dosed at 20 mg/kg in a BxPC3 pancreatic tumor xenograft model in CB 17 SCID mice (vehicle control, PBS (♦)).

Figure 8 is a graph summarizing results from an experiment to measure the tumor inhibitory activity of the anti-ErbB3 antibodies 04D01 (Δ), 09D03 (*), 11G01 (□), 12A07 (▴), 18H02 (▯), 22A02 (▪), 24C05 (◯) and a human IgG control (- -▪- -) dosed at 20 mg/kg in a BxPC3 pancreatic tumor xenograft model in CB 17 SCID mice (vehicle control, PBS (♦)).


Specifications

  • Immunogen
  • Recombinant human ErbB3/Fc
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2b, κ
  • Specificity
  • Human ERBB3
  • Species Reactivity
  • Human
  • Clone
  • 09D03
  • Applications
  • ELISA, Neut

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for ELISA, Neutralization and Function Assay. For details, refer to Published Data.

Target

  • Alternative Names
  • Erb-B2 Receptor Tyrosine Kinase 3; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 3; Tyrosine Kinase-Type Cell Surface Receptor HER3; Human Epidermal Growth Factor Receptor 3; Proto-Oncogene-Like Protein C-ErbB-3; EC 2.7.10.1; HER3; Lethal Congenital Contracture Syndrome 2; Receptor Tyrosine-Protein Kinase ErbB-3; P180-ErbB3;

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone 09D03"

See other products for "ERBB3"

Humanized Antibody

CAT Product Name Application Type
TAB-892 Anti-Human ERBB3/ErbB 3 Recombinant Antibody (Seribantumab) IF, IP, Neut, FuncS, ELISA, FC, ICC IgG2 - lambda
TAB-057CT Human Anti-ERBB3 Recombinant Antibody (TAB-057CT) Inhibion, ELISA Humanized antibody
TAB-058CT Human Anti-ERBB3 Recombinant Antibody (TAB-058CT) ELISA, Inhib, Cty, IHC, WB, FC Human IgG1, κ
TAB-057CT-F(E) Human Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-057CT-F(E)) Inhibion, ELISA Humanized Fab
TAB-058CT-F(E) Human Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-058CT-F(E)) WB, ELISA, Inhib Humanized Fab

Immunotoxin

CAT Product Name Application Type
AGTO-L074E HRGβ2-PE immunotoxin Cytotoxicity assay, Functional assay

Recombinant Antibody

Chimeric Antibody

CAT Product Name Application Type
TAB-061CT Human Anti-ERBB3 Recombinant Antibody (TAB-061CT) Inhib, ELISA Chimeric (Rabbit/Human) antibody
TAB-061CT-S(P) Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-061CT-S(P)) Inhib, ELISA Human scFv
TAB-061CT-F(E) Human Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-061CT-F(E)) Inhib, ELISA Chimeric (Rabbit/Human) Fab

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1179 Hi-Affi™ Rabbit Anti-ERBB3 Recombinant Antibody (clone DS1179AB) ICC, IF, WB Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H21 Afuco™ Anti-ERBB3 ADCC Recombinant Antibody (Duligotumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody
AFC-TAB-422CQ Afuco™ Anti-ERBB3 ADCC Recombinant Antibody (Elgemtumab), ADCC Enhanced ELISA, IHC, FC, IP, IF, BL ADCC enhanced antibody
AFC-TAB-189 Afuco™ Anti-ERBB3 Recombinant Antibody (AFC-TAB-189), ADCC Enhanced IP, IF, FuncS, FC, Neut, ELISA Human IgG1, κ
AFC-TAB-H22 Afuco™ Anti-ERBB3 ADCC Recombinant Antibody (Duligotuzumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody
AFC-TAB-892 Afuco™ Anti-ERBB3 ADCC Recombinant Antibody (Seribantumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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