Rabbit Anti-ERLIN2 Recombinant Antibody (clone R42-7O-2) (CAT#: VS3-FY2148)
Clone R42-7O-2 is a rabbit recombinant antibody that is designed to recognize human ERLIN2 protein, a calculated weight of 38 kDa. It cross-reacts with mouse.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R42-7O-2 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/if_selected.jpg
Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/14894/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/14894/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/7619_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/7619_B_8_5.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/7619_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/7619_A_7_3.jpg
Figure 5 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/7619_A_7_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/7619_A_7_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/7619_A_9_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/7619_A_9_5.jpg
Figure 7 Testis
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/7619_A_6_6.jpg
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/7619_A_6_6.jpg
Figure 8 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/7619_A_8_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2025/7619_A_8_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147475-ERLIN2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147475-ERLIN2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse ERLIN2
- Species Reactivity
- Human, Mouse
- Clone
- R42-7O-2
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 38 kDa, while the observed molecular weight stands at 38kDa.
- Related Disease
- Spastic Paraplegia 18B, Autosomal Recessive and Spastic Paraplegia 18A, Autosomal Dominant
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- ER Lipid Raft Associated 2; Stomatin-Prohibitin-Flotillin-HflC/K Domain-Containing Protein 2; Endoplasmic Reticulum Lipid Raft-Associated Protein 2; Spastic Paraplegia 18 (Autosomal Dominant); SPFH Domain Family, Member 2; C8orf2; SPFH2
- Gene ID
- 11160
- UniProt ID
- O94905
- Sequence Similarities
- Belongs to the band 7/mec-2 family.
- Cellular Localization
- Endoplasmic reticulum, Membrane
- Post Translation Modifications
- Glycosylation at Asn106, Thr334, and Thr336 (NX_O94905 [NX_O94905-1])
Modification sites at PhosphoSitePlus (O94905)
Glycosylation from GlyConnect ERLN2_HUMAN (1222)
Glycosylation from GlyGen (O94905) 5 sites, 10 N-linked glycans (1 site), 2 O-linked glycans (4 sites)
- Protein Refseq
- XP_047277263.1; XP_047277264.1; XP_054215652.1; XP_054215653.1; NP_001003790.1
- Function
- Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1.
Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1192 | Hi-Affi™ Rabbit Anti-ERLIN2 Recombinant Antibody (clone DS1192AB) | WB, IHC, ICC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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