Mouse Anti-Estradiol Recombinant Antibody (clone 8D9) (CAT#: FAMAB-0134CQ)

Figure 1 Structural specificity of the anti-oestradiol antibody on enhancing the CK response to E₂ in vivo.

Ovariectomized female rats were injected with mouse IgG2b (mouse IgG, 25 μg/rat), E₂ (5 μg/rat), anti-E2 15 (25 μg/rat), anti-E2 11B₆ (25 μg/rat), anti-E2 8D₉ (25 μg/rat), the Fab' of anti-E2 15 (25 μg/rat) or anti-oestradiol antibody plus E₂ (clone 15, 11B₆ or 8D₉). The animals were killed 24 h later, the various organs removed and the CK specific activity assayed. Di, diaphysis; Ep, epiphysis, Ut, uterus. Results are expressed as mean±S.E.M. of the ratios of experimental/control (E/C) where the control is the value of the various treatments in the absence of E2 for n = 5-10. *P < 0.05, **P < 0.01 for the statistical significance compared to untreated control group. ##For the statistical significance between Ab15 + E₂ vs E₂.

Sömjen, D., Amir-Zaltsman, Y., Mor, G., Gayer, B., Lichter, S., Nevo, N., & Kohen, F. (1998). A monoclonal antibody to oestradiol potentiates the stimulation of the specific activity of the brain type creatine kinase by oestrogen in vivo and in vitro. The Journal of steroid biochemistry and molecular biology, 64(5-6), 297-304.