Rabbit Anti-EZH2 Recombinant Antibody (clone 2C7)
CAT#: ZG-0658J
This product is a rabbit antibody that recognizes EZH2.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-EZH2 Antibody (ZG-0658J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/2008_C2_16_cr5f1a9e9d6c53d_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/2027_D2_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/582_H8_1_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106462-EZH2
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Specifications
- Immunogen
- A synthesized peptide derived from human KMT6 / EZH2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human EZH2
- Species Reactivity
- Human
- Clone
- 2C7
- Applications
- ELISA, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
IHC:1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "EZH2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3981z | Mouse Anti-EZH2 Recombinant Antibody (clone 21F1) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| ZG-0657J | Rabbit Anti-EZH2 Recombinant Antibody (clone 10H8) | ELISA, IHC | Rabbit IgG |
| VS3-XY657 | Mouse Anti-EZH2 Recombinant Antibody (clone 9A11B9) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY526 | Recombinant Rabbit Anti-EZH2 Antibody (clone R07-5B1) | WB, ICC, IF | Rabbit IgG |
| VS13-YC368 | Rabbit Anti-EZH2 Recombinant Antibody (VS13-YC368) | IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC078 | PE-A*02:01/Human EZH2 (YMCSFLFNL) MHC Tetramer | FCM | |
| MHC-CN0657 | PE-A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0658 | APC-A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0659 | FITC-A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0660 | A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1221 | Hi-Affi™ Rabbit Anti-EZH2 Recombinant Antibody (clone DS1221AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY197 | Mouse Anti-NHP EZH2 Recombinant Antibody (clone H.547.3) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2396 | Anti-EZH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2397 | Anti-Mouse EZH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2398 | Anti-Rat EZH2 Immunohistochemistry Kit | IHC |
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