Rabbit Anti-F11R Recombinant Antibody (VS13-YC369)

Name: Rabbit Anti-F11R Recombinant Antibody (VS13-YC369)
SKU: VS13-YC369
Rating: 5 (1 reviews)

CAT#: VS13-YC369

The Rabbit Anti-F11R Recombinant Antibody is specific for human F11R. F11R, expressed in junctions like tight junctions, regulates cell adhesion and impacts immunity pathways, illustrated by its receptor functions in infection and platelet interactions. The anti-F11R antibody can be used for Western blot (1/500-1/1000), Immunofluorescence (1/50-1/200), Immunoprecipitation (1/20).

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cell junctions & microtubules.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43616/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line CACO-2

Immunofluorescent staining of human cell line CACO-2 shows localization to microtubules.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43616/1026_E7_1_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line MCF7

Immunofluorescent staining of human cell line MCF7 shows localization to microtubules.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43616/965_H10_1_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_B_8_5.jpg

Normal Tissue Figure 5 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_A_9_3.jpg

Normal Tissue Figure 6 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_A_8_4.jpg

Normal Tissue Figure 7 Kidney

Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining:Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_A_7_5.jpg

Normal Tissue Figure 8 Testis

Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_A_6_6.jpg

Normal Tissue Figure 9 Skin

Epidermal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_B_4_8.jpg

Normal Tissue Figure 10 Lymph node

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_A_8_8.jpg

RNA Expression Figure 11 RNA cell line category: Cell line enhanced (HaCaT, OE19, SuSa)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000158769-F11R

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Specifications

Immunogen
Recombinant human junctional adhesion molecule 1.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human F11R

Species Reactivity
Human

Applications
WB, ICC, IF, IP

Conjugate
NONE

Related Disease
Cardiovascular Disease

Product Property

Clonality
Monoclonal

Purification
Affinity Chromatography purified

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.

Preservative
0.01% Sodium azide

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Shipping with ice pack.

Applications

Application Notes
Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/20

Target

Alternative Names
F11 Receptor; Junctional Adhesion Molecule 1; Platelet Adhesion Molecule 1; Platelet F11 Receptor; PAM-1; JAM1; JCAM; Junctional Adhesion Molecule A;

Gene ID
50848

UniProt ID
Q9Y624

Long Name
F11 Receptor

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS13-YC369.pdf

MSDS

MSDS - VS13-YC369.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

CAT
Product Name

Isotype Control

CAT
Product Name

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NAB-914-VHH	Recombinant Anti-Human F11R VHH Single Domain Antibody	ICC, FC, WB, FUNC	Llama VHH

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TAB-0606CL	Anti-Human JAM-A Recombinant Antibody (TAB-0606CL)	IF, IHC, FC, WB
TAB-0606CL-S(P)	Anti-Human F11R Recombinant Antibody scFv Fragment (6F4)	ELISA
TAB-0606CL-F(E)	Anti-Human F11R Recombinant Antibody Fab Fragment (6F4)	ELISA

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MOB-2149MZ	Recombinant Mouse Anti-Human F11R Antibody (clone N.Bc.G22)	FC, IP, WB	Mouse antibody
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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
HPAB-0298-CN-S(P)	Human Anti-JAM-A Recombinant Antibody; scFv Fragment (HPAB-0298-CN-S(P))	ELISA, WB, IF	Humanized scFv
HPAB-0137-FY-S(P)	Recombinant Human Anti-F11R Antibody scFv Fragment (Hz6F4-2)	In vivo activity	Human scFv
HPAB-0880-FY-F(E)	Mouse Anti-F11R Recombinant Antibody (clone 6F4); scFv Fragment	Activ	Humanized scFv

CAT	Product Name	Application	Type
HPAB-0297-CN-F(E)	Human Anti-JAM-A Recombinant Antibody; Fab Fragment (HPAB-0297-CN-F(E))	ELISA, WB, IF	Chimeric (mouse/human) Fab
HPAB-0298-CN-F(E)	Human Anti-F11R Recombinant Antibody; Fab Fragment (HPAB-0298-CN-F(E))	ELISA, WB, IF	Humanized Fab
HPAB-0137-FY-F(E)	Recombinant Human Anti-F11R Antibody Fab Fragment (Hz6F4-2)	In vivo activity	Human Fab, κ
HPAB-0171CQ-F(E)	Rat Anti-F11r Recombinant Antibody (clone MAb BV12); Fab Fragment	WB, IP, IF	Rat Fab
HPAB-0880-FY-S(P)	Mouse Anti-F11R Recombinant Antibody (clone 6F4); Fab Fragment	Activ	Humanized Fab

CAT	Product Name	Application	Type
VS-0724-YC1455	AbPlus™ Anti-F11R Magnetic Beads (VS-0724-YC1455)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0225-XY110	CytoStream™ Rat Anti-F11R Recombinant Antibody (clone H202-106)	FC	Rat IgG1, kappa

CAT	Product Name	Application	Type
VS-0325-FY20	Human Anti-F11R (clone 6F4) scFv-Fc Chimera	ELISA	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC325	Recombinant Anti-F11R Vesicular Antibody, EV Displayed (VS-0425-YC325)	ELISA, FC, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-XY2402	Anti-F11R Immunohistochemistry Kit	IHC
VS-0525-XY2403	Anti-Mouse F11R Immunohistochemistry Kit	IHC

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