Human Anti-F8 Recombinant Antibody (clone KM33) (CAT#: FAMAB-0197-CN)

Figure 1 fVIII functional assays in the presence of group A, AB, and B anti-C1 mAbs.

(A) Inhibition of fVIII procoagulant activity by type 2 group A mAb I84 (●), type 1 group AB mAb KM33 (▪), and type 1 group B mAb B136 (△) by Bethesda assay. (B) Inhibition of fVIII binding to PCPS and (C) fVIII binding to VWF in the presence of anti-C1 mAbs group A B153 (●), group AB KM33 (▪), and group B B136 (△) determined by competitive ELISAs.

Batsuli, G., Deng, W., Healey, J. F., Parker, E. T., Baldwin, W. H., Cox, C., ... & Lollar, P. (2016). High-affinity, noninhibitory pathogenic C1 domain antibodies are present in patients with hemophilia A and inhibitors. Blood, 128(16), 2055-2067.

Figure 2 Inhibition of uptake of fVIII by dendritic cells by anti-C1 mAbs.

Flow cytometric histograms of 10 nM DyLight 650-conjugated recombinant full-length fVIII uptake by human MDDCs in the absence and presence of 80 nM group AB KM33. Representative histograms of untreated (gray) and treated (white) MDDCs displaying the percentage of internalized fVIII in the absence or presence of the anti-C1 mAbs tested are shown. Percentages of fVIII uptake shown in the insets were estimated by the integrated fluorescence beyond the control histogram.

Batsuli, G., Deng, W., Healey, J. F., Parker, E. T., Baldwin, W. H., Cox, C., ... & Lollar, P. (2016). High-affinity, noninhibitory pathogenic C1 domain antibodies are present in patients with hemophilia A and inhibitors. Blood, 128(16), 2055-2067.

Figure 3 Inhibition of uptake of fVIII by dendritic cells by anti-C1 mAbs.

Median fluorescence intensities of human MDDCs incubated with increasing concentrations of DyLight 650-conjugated fVIII alone (control) and in the presence of mAbs 2A9, F156, I84, KM33, and B136.

Batsuli, G., Deng, W., Healey, J. F., Parker, E. T., Baldwin, W. H., Cox, C., ... & Lollar, P. (2016). High-affinity, noninhibitory pathogenic C1 domain antibodies are present in patients with hemophilia A and inhibitors. Blood, 128(16), 2055-2067.