Mouse Anti-FANCM Recombinant Antibody (clone CV5.1) (CAT#: FAMAB-1133CQ)
This product is a recombinant mouse antibody that recognizes FANCM. The antibody was purified by affinity chromatography. It can be used in ICC, WB, IF, IHC.
Specific Inquiry
IF
Figure 1 FANCM suppresses telomeric DNA damage and localizes to telomeres in ALT cells.
Examples of Ps33 combined with TRF2 immunostaining on U2OS and HeLa cells transfected with the indicated siRNAs and harvested 48 h after transfection.
Silva, B., Pentz, R., Figueira, A. M., Arora, R., Lee, Y. W., Hodson, C., ... & Azzalin, C. M. (2019). FANCM limits ALT activity by restricting telomeric replication stress induced by deregulated BLM and R-loops. Nature communications, 10(1), 1-16.
WB
Figure 2 BLM depletion substantially averts the phenotypes associated with FANCM depletion.
Western blot analysis of FANCM and BLM in U2OS cells transfected with siFa.
Silva, B., Pentz, R., Figueira, A. M., Arora, R., Lee, Y. W., Hodson, C., ... & Azzalin, C. M. (2019). FANCM limits ALT activity by restricting telomeric replication stress induced by deregulated BLM and R-loops. Nature communications, 10(1), 1-16.
FC
Figure 3 FANCM supports normal cell-cycle progression and proliferation of ALT cells.
Examples of FACS profiles of the indicated siRNAtransfected cells stained with propidium iodide (PI). Cell counts (y axis) are plotted against PI intensity (x axis). Cells were harvested 48 h after
transfection.
Silva, B., Pentz, R., Figueira, A. M., Arora, R., Lee, Y. W., Hodson, C., ... & Azzalin, C. M. (2019). FANCM limits ALT activity by restricting telomeric replication stress induced by deregulated BLM and R-loops. Nature communications, 10(1), 1-16.
Specifications
- Immunogen
- Denatured FANCM (aa 1507-1679)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human FANCM
- Species Reactivity
- Human
- Clone
- CV5.1
- Applications
- IF, WB, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunofluorescence, Western Blot and Flow Cytometry.
Target
- Alternative Names
- Fanconi Anemia Complementation Group M; KIAA1596; Fanconi Anemia-Associated Polypeptide Of 250 KDa; ATP-Dependent RNA Helicase FANCM; Protein Hef Ortholog; FAAP250
- Gene ID
- 57697
- UniProt ID
- Q8IYD8
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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