Human Anti-FASLG Recombinant Antibody (clone huF919G1V2) (CAT#: VS-0724-YC4)

• Host Species
• Human

• Derivation
• Humanized

• Type
• Human IgG1 kappa

• Specificity
• Human Fas ligand

• Species Reactivity
• Human

• Clone
• huF919G1V2

• Applications
• ELISA, WB, FC, Apop

• Purification
• Protein A purified

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Buffer
• PBS, pH 7.4

• Preservative
• No preservatives

• Storage
• Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Flow cytometry: 0.01-50 μg/mL

• Alternative Names
• APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; TNLG1A; APT1LG1; tumor necrosis factor ligand superfamily member 6; CD95 ligand; Fas ligand (TNF superfamily, member 6); apoptosis (APO-1) antigen ligand 1; apoptosis antigen ligand; fas antigen ligand; mutant tumor necrosis factor family member 6; tumor necrosis factor ligand 1A

• Gene ID
• 356

• UniProt ID
• P48023

• Long Name
• Fas Ligand

• Sequence Similarities
• Belongs to the tumor necrosis factor family.

• Cellular Localization
• Cell membrane; Single-pass type II membrane protein
  Cytoplasmic vesicle lumen
  Lysosome lumen
  [FasL intracellular domain]
  Nucleus
  [Tumor necrosis factor ligand superfamily member 6, soluble form]
  Secreted

• Post Translation Modifications
• The soluble form derives from the membrane form by proteolytic processing.
  The membrane-bound form undergoes two successive intramembrane proteolytic cleavages.
  The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain.
  This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD).
  FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.
  N-glycosylated.
  Glycosylation enhances apoptotic activity.
  Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.
  Glycosylation at Asn184, Asn250, and Asn260.

• Protein Refseq
• NP_000630.1; NP_001289675.1

• Function
• Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells.
  Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development.
  Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses.
  TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity).
  Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis.
  [Tumor necrosis factor ligand superfamily member 6, soluble form]: Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways.
  Can induce apoptosis but does not appear to be essential for this process.
  [FasL intracellular domain]: Cytoplasmic form induces gene transcription inhibition. TNFL6_HUMAN,P48023

Isotype Control

• MOB-0402ZL
• Human IgG1, kappa - Isotype Control Antibody

Secondary Antibody

• 2ND-024CQ
• Goat Anti-Human IgG (H&L) Antibody

• 2ND-025CQ
• Rabbit Anti-Human IgG (H&L) Antibody

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