Mouse Anti-Fibronectin Recombinant Antibody (clone FDB3) (CAT#: HPAB-0015LY)

This product is antibody recognizes fibronectin. The antibody FDB3 can be used in some immunoassay techniques such as: WB.


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IF

Figure 1 Effect of III₁c on fibronectin matrix morphology.

Figure 1 Effect of III₁c on fibronectin matrix morphology.

Fibroblasts were cultured on glass coverslips in the presence of fibronectin conjugated with Alexa Fluor-488 (10 μg/ml), indicated as AF-FN, for three days. Wells were washed and incubated with 20 μM III1c or carrier buffer as control for 20 hours. Cells were washed, fixed, permeabilized and stained for fibronectin using the designated mAbs. Alexa Fluor-488 labeled fibronectin was visualized and total matrix fibronectin was detected by indirect immunofluorescence using either an antibody against the CS domain (FDB3) or an antibody against the EDA domain (IST-9). The figure represents one experiment repeated three times.

Klein, & R., M. . (2003). Stimulation of extracellular matrix remodeling by the first type iii repeat in fibronectin. Journal of Cell Science, 116(22), 4663-4674.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Clone
  • FDB3
  • Applications
  • WB

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS, 150 mM NaCl, 0.01% BSA, pH 6.0
  • Preservative
  • 0.05% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The fibronectin antibody has been reported in applications of Western Blot, Immunofluorescence.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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See other products for "Clone FDB3"

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Recombinant Antibody

CAT Product Name Application Type
MOB-361 Recombinant Anti-fibronectin Antibody WB, ELISA, IHC, FuncS IgG
MHH-361 Recombinant Human Anti-fibronectin Antibody WB, Neut, FuncS IgG

Fab Fragment Antibody

scFv Fragment Antibody

CAT Product Name Application Type
MOB-361-S(P) Recombinant Anti-fibronectin Antibody scFv Fragment ELISA, WB, IP, FuncS scFv
MHH-361-S(P) Recombinant Human Anti-fibronectin Antibody scFv Fragment IP, IF, Biosensors, FuncS scFv

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0970MZ Chicken Anti-Fibronectin Polyclonal IgY ELISA, WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4177 Hi-Affi™ Rabbit Anti-Fibronectin Recombinant Antibody (clone SI254DS) ELISA, WB, IP Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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