Rabbit Anti-FLT1 Recombinant Antibody (VS3-CJ732)
CAT#: VS3-CJ732
This product is a rabbit antibody that recognizes human, mouse, and rat FLT1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/90_G3_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/1368_A5_2_blue_green.jpg
Normal Tissue
Figure 3 Testis
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/142518_A_6_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, HMC-1, HUVEC TERT2, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102755-FLT1
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat FLT1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- FLT; FLT-1; VEGFR1; VEGFR-1
- Gene ID
- 2321
- UniProt ID
- P17948
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Endosome, Membrane, Secreted
- Post Translation Modifications
- N-glycosylated.
Ubiquitinated after VEGFA-mediated autophosphorylation, leading to proteolytic degradation.
Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-1169 is important for interaction with PLCG. Phosphorylation at Tyr-1213 is important for interaction with PIK3R1, PTPN11, GRB2, and PLCG. Phosphorylation at Tyr-1333 is important for endocytosis and for interaction with CBL, NCK1 and CRK. Is probably dephosphorylated by PTPRB.
- Protein Refseq
- NP_001153392.1; NP_001153502.1; NP_001153503.1
- Function
- Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Likewise, isoforms lacking a transmembrane domain, such as isoform 2, isoform 3 and isoform 4, may function as decoy receptors for VEGFA. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1. Isoform 1 phosphorylates PLCG. Isoform 2-4:
May function as decoy receptor for VEGFA. Isoform 7 has a truncated kinase domain; it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion.
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Related Diseases
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| TAB-009CQ | Human Anti-FLT1 Recombinant Antibody (TAB-009CQ) | ELISA | Chimeric antibody (mouse/human) |
| TAB-009CQ-S(P) | Mouse Anti-FLT1 Recombinant Antibody; scFv Fragment (TAB-009CQ-S(P)) | ELISA | Mouse scFv |
| TAB-009CQ-F(E) | Human Anti-FLT1 Recombinant Antibody; Fab Fragment (TAB-009CQ-F(E)) | ELISA | Chimeric antibody (mouse/human) |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0785-CN-S(P) | Mouse Anti-FLT1 Recombinant Antibody (clone 1-833-527); scFv Fragment | ELISA, FC | Mouse scFv |
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| HPAB-0500-YJ-S(P) | Human Anti-FLT1 Recombinant Antibody; scFv Fragment (HPAB-0500-YJ-S(P)) | ELISA, Block, FuncS | Human scFv |
| HPAB-0501-YJ-S(P) | Mouse Anti-FLT1 Recombinant Antibody; scFv Fragment (HPAB-0501-YJ-S(P)) | ELISA, Block, FuncS | Mouse scFv |
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| MHC-CN0673 | PE-A*02:01/Human FLT1 (TLFWLLTL) MHC Tetramer | FCM, IHC | |
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| MHC-CN0675 | FITC-A*02:01/Human FLT1 (TLFWLLTL) MHC Tetramer | FCM, IHC | |
| MHC-CN1412 | PE-A*24:02/Human FLT1 (SYGVLLWEI) MHC Tetramer | FCM | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| HPAB-1700-FY | Mouse Anti-FLT1 Recombinant Antibody (clone CA0071-Z2ZA-01171) | ELISA | Mouse IgG |
| HPAB-1701-FY | Mouse Anti-FLT1 Recombinant Antibody (clone CA0071-Z2ZB-01171) | ELISA | Mouse IgG |
| HPAB-1702-FY | Human Anti-FLT1 Recombinant Antibody (clone 6F9) | ELISA, WB | Human IgG1 |
| HPAB-1703-FY | Human Anti-FLT1 Recombinant Antibody (clone 13G12) | ELISA, WB | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1699-FY-S(P) | Mouse Anti-FLT1 Recombinant Antibody (clone CA0071-Z2ZM-01941); Fab Fragment | ELISA | Mouse Fab |
| HPAB-1700-FY-S(P) | Mouse Anti-FLT1 Recombinant Antibody (clone CA0071-Z2ZA-01171); Fab Fragment | ELISA | Mouse Fab |
| HPAB-1701-FY-S(P) | Mouse Anti-FLT1 Recombinant Antibody (clone CA0071-Z2ZB-01171); Fab Fragment | ELISA | Mouse Fab |
| HPAB-1702-FY-S(P) | Human Anti-FLT1 Recombinant Antibody (clone 6F9); Fab Fragment | ELISA, WB | Human Fab |
| HPAB-1703-FY-S(P) | Human Anti-FLT1 Recombinant Antibody (clone 13G12); Fab Fragment | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
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| VS-0325-XY850 | Anti-FLT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2581 | Anti-Mouse FLT1 Immunohistochemistry Kit | IHC | |
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