Mouse Anti-FLT3 Recombinant Antibody (clone BV10-A4H2) (CAT#: VS3-CJ918)

• Immunogen
• BV-173 pro-B cell line

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Human FLT3 (CD135)

• Species Reactivity
• Human

• Clone
• BV10-A4H2

• Applications
• IF, FC

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS, pH 7.3.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C for one year. Do not freeze.

• Shipping
• Shipped at 4°C

• Application Notes
• This antibody has been tested for use in Immunofluorescence, Flow Cytometry.

• Alternative Names
• FLK2; STK1; CD135; FLK-2

• Gene ID
• 2322

• UniProt ID
• P36888

• Sequence Similarities
• Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

• Cellular Localization
• Endoplasmic reticulum, Membrane

• Post Translation Modifications
• N-glycosylated, contains complex N-glycans with sialic acid.
  Autophosphorylated on several tyrosine residues in response to FLT3LG binding. FLT3LG binding also increases phosphorylation of mutant kinases that are constitutively activated. Dephosphorylated by PTPRJ/DEP-1, PTPN1, PTPN6/SHP-1, and to a lesser degree by PTPN12. Dephosphorylation is important for export from the endoplasmic reticulum and location at the cell membrane.
  Rapidly ubiquitinated by UBE2L6 and the E3 ubiquitin-protein ligase SIAH1 after autophosphorylation, leading to its proteasomal degradation.

• Protein Refseq
• NP_004110.2

• Function
• Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS