Human Anti-FLT3 Recombinant Antibody (clone NC7) (CAT#: HPAB-0183CQ)
This product is a recombinant human antibody that recognizes FLT3. This product is a fully human antibody that specifically bind to human FLT3 within extracellular domains 5 with high affinity.
Specific Inquiry
WB
Figure 1 FLT3 Antibody (HPAB-0183CQ) in WB
Western blot analysis was performed by loading 1 µg (lane 1) and 1 µg (lane 2) recombinant human FLT3 antigen onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with TLT3 antibody (HPAB-0183CQ) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
ELISA
Figure 2 FLT3 Antibody (HPAB-0183CQ) in ELISA
ELISA analysis of HPAB-0183CQ was performed by coating with recombinant human FLT3 antigen. Then blocked with BSA, and incubated with TLT3 antibody (HPAB-0183CQ) at a starting concentration of 1µg/mL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
ELISA
Figure 1 Anti-FLT3 Antibody (HPAB-0183CQ) in ELISA
ELISA analysis of HPAB-0183CQ was performed by coating with FLT3 Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
Specifications
- Host Species
- Human
- Type
- Human IgG1, κ
- Specificity
- Human FLT3
- Species Reactivity
- Human
- Clone
- NC7
- Applications
- ELISA, Neut
- Related Disease
- Acute myeloid leukemia
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Blocking and Functional Assay. Please see the Published Data for further information.
Target
- Alternative Names
- Fms Related Tyrosine Kinase 3; Stem Cell Tyrosine Kinase 1; Fms-Like Tyrosine Kinase 3; FL Cytokine Receptor; CD135 Antigen; EC 2.7.10.1; CD135; FLK-2; STK1; FLK2;
- Gene ID
- 2322
- UniProt ID
- P36888
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1630z | Mouse Anti-FLT3 Recombinant Antibody (clone 23F1) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| MOB-0606CT | Recombinant Mouse anti-Human FLT3 Monoclonal antibody (CW20B5) | FC, IP | |
| HPAB-0103-YC | Human Anti-FLT3 Recombinant Antibody (HPAB-0103-YC) | ELISA, Block, FC | Humanized IgG1, κ |
| HPAB-0182CQ | Human Anti-FLT3 Recombinant Antibody (clone D4-3) | ELISA, Neut | Human IgG1, κ |
| VS3-XY714 | Mouse Anti-FLT3 Recombinant Antibody (clone 7B7C3) | ELISA, WB, IHC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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