Human Anti-FN1 Recombinant Antibody (clone Fn52)
CAT#: VS-0623-WK13
The antibody clone Fn52 was produced by immunizing with purified human Fibronectin. It can be used for detection of human Fibronectin in ELISA, WB and IF staining.
Gene Expression
Published Data
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_B_9_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_4.jpg
Normal Tissue
Figure 4 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_5_6.jpg
Normal Tissue
Figure 5 Placenta
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_1_7.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC TERT1, HSkMC, hTERT-HME1, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115414-FN1
❮
❯
WB
Figure 1 Western blot images of full length fibronectin with scFv Fn52.
Full length fibronectin using anti-fibronectin antibody (lane 1); scFv Fn52 as primary antibody for full length fibronectin (lane 2), 70 kDa fragment of fibronectin(lane 3) and the 30 kDa fragment of fibronectin (lane 4) respectively.
RPE Cell Migration Assay
Figure 2 Fn52 and Fn52RGDS inhibit the migration of D407 RPE cells.
RPE cells were pre-incubated for 1 h with the various reagents, at indicated concentrations, and added to the upper chamber of the transwell insert, while the bottom chamber in each well, contained Fn at a concentration of 20 mg/ml (control).
Fibronectin Assembly Assay
Figure 3 Fn52 and Fn52RGDS inhibit the fibronectin polymerization.
Immunocytochemistry of ARPE-19 retinal pigment epithelial cells, cultured in the presence of patient-derived vitreous and subretinal fluid, in the presence and absence of scFv antibodies.
MMP Activity Assay
Figure 4 Fn52 and Fn52RGDS inhibit the MMP expression in ARPE-19 RPE cells.
lane 1: no scFv; lane 2: scFvO27; lane 3: scFv Fn52; lane 4: scFv Fn52RGDS.
❮
❯
Specifications
- Immunogen
- Purified human Fibronectin
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human Fibronectin
- Species Reactivity
- Human
- Clone
- Fn52
- Applications
- ELISA, WB, FuncS, RPE Cell Migration Assay, Fibronectin Assembly Assay, MMP Activity Assay
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western blot: 1/400-1/1000
FuncS: 10 μg/mL-25 μg/mL
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0623-WK13. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
AGE-RAGE Signaling Pathway in Diabetic Complications
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "FN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1294z | Mouse Anti-FN1 Recombinant Antibody (clone 6H4) | IHC, IP, WB | Mouse IgG2b, κ |
| MOB-1516MZ | Recombinant Mouse Anti-Human FN1 Antibody (clone 3G23) | ELISA, WB | Mouse antibody |
| FAMAB-0105YC | Human Anti-FN1 Recombinant Antibody (clone ME4C) | ELISA, IHC | Human IgG |
| VS3-WK1282 | Mouse Anti-FN1 Recombinant Antibody (VS3-WK1282) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-WK1577 | Mouse Anti-FN1 Recombinant Antibody (clone 6C10) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-071WM | Anti-Human FN1 Recombinant Antibody (CGS-1) | ELISA, WB | Human Antibody |
| TAB-001CQ-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (MOR03255) | ELISA, BL, IHC | Human antibody |
| TAB-002CQ-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (ME-4C) | ELISA, IHC | Human antibody |
| TAB-003CQ-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (1C5) | ELISA, WB | Human antibody |
| TAB-004CQ-F(E) | Human Anti-FN1 Recombinant Antibody; Fab Fragment (TAB-004CQ-F(E)) | ELISA, IHC, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-852CQ | Mouse Anti-FN1 Recombinant Antibody (clone HFN7.1) | ELISA, WB, IP, Block | Mouse IgG1 |
| NEUT-853CQ | Mouse Anti-FN1 Recombinant Antibody (clone IST-9) | WB, RIA, ELISA, IHC-Fr, ICC, IF, Block, IHC-P | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1326 | Hi-Affi™ Rabbit Anti-FN1 Recombinant Antibody (clone DS1326AB) | IHC-Fr, IHC-P, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0105-YC-S(P) | Human Anti-FN1 Recombinant Antibody (clone ME4C); scFv Fragment | ELISA, IHC | Human scFv |
| HPAB-1110-FY-F(E) | Human Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-1110-FY-F(E)) | IHC | Human Fab |
| HPAB-1705-FY-F(E) | Mouse Anti-FN1 Recombinant Antibody (clone MOR02610); scFv Fragment | ELISA, IHC | Mouse scFv |
| HPAB-1706-FY-F(E) | Mouse Anti-FN1 Recombinant Antibody (clone MOR02611); scFv Fragment | ELISA, IHC | Mouse scFv |
| HPAB-1707-FY-F(E) | Mouse Anti-FN1 Recombinant Antibody (clone MOR02613); scFv Fragment | ELISA, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1110-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1110-FY-S(P)) | IHC | Human scFv |
| HPAB-1111-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1111-FY-S(P)) | IHC | Human scFv |
| HPAB-1112-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1112-FY-S(P)) | IHC | Human scFv |
| HPAB-1113-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1113-FY-S(P)) | IHC | Human scFv |
| HPAB-1114-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1114-FY-S(P)) | IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NBD-0040-YJ-VHH | Camelid Anti-FN1 Recombinant Single Domain Antibody (NBD-0040-YJ-VHH) | ELISA, In vivo imaging | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY105 | AbPlus™ Anti-FN1 Magnetic Beads (HFN7.1) | IP, Protein Purification | |
| VS-0724-YC853 | AbPlus™ Anti-FN1 Magnetic Beads (VS-0724-YC853) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY204 | Mouse Anti-NHP FN1 Recombinant Antibody (clone C6F10) | WB, IHC, IF, IP | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY108 | Human Anti-FN1 (clone L19) scFv-Fc Chimera | IHC, ELISA, IF | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2593 | Anti-FN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2594 | Anti-Rat FN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2595 | Anti-Mouse FN1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC87 | Recombinant Anti-FN1 ED-B (N-terminus x C-terminus) Biparatopic Antibody, Tandem scFv (Clone CGS-2 x Clone CGS-1) | ELISA | Tandem scFv |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, ELISA, FuncS
-2.png)
Application: IB, ELISA, FC, FuncS
Application: FC, FRET, Internalization
Application: WB, ELISA, IF, FuncS, Apop, BA
Application: ELISA, IHC, FC, IP, IF, Inhib
-3.jpg)
Application: Neut
Application: ELISA, Inhib, FuncS
Application: WB, FC, IF, Inhib, ELISA, IHC
-2.png)
Application: ELISA
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
