Human Anti-FN1 Recombinant Antibody (clone Fn52) (CAT#: VS-0623-WK13)
The antibody clone Fn52 was produced by immunizing with purified human Fibronectin. It can be used for detection of human Fibronectin in ELISA, WB and IF staining.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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WB
Figure 1 Western blot images of full length fibronectin with scFv Fn52.
Full length fibronectin using anti-fibronectin antibody (lane 1); scFv Fn52 as primary antibody for full length fibronectin (lane 2), 70 kDa fragment of fibronectin(lane 3) and the 30 kDa fragment of fibronectin (lane 4) respectively.
RPE Cell Migration Assay
Figure 2 Fn52 and Fn52RGDS inhibit the migration of D407 RPE cells.
RPE cells were pre-incubated for 1 h with the various reagents, at indicated concentrations, and added to the upper chamber of the transwell insert, while the bottom chamber in each well, contained Fn at a concentration of 20 mg/ml (control).
Fibronectin Assembly Assay
Figure 3 Fn52 and Fn52RGDS inhibit the fibronectin polymerization.
Immunocytochemistry of ARPE-19 retinal pigment epithelial cells, cultured in the presence of patient-derived vitreous and subretinal fluid, in the presence and absence of scFv antibodies.
MMP Activity Assay
Figure 4 Fn52 and Fn52RGDS inhibit the MMP expression in ARPE-19 RPE cells.
lane 1: no scFv; lane 2: scFvO27; lane 3: scFv Fn52; lane 4: scFv Fn52RGDS.
Specifications
- Immunogen
- Purified human Fibronectin
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human Fibronectin
- Species Reactivity
- Human
- Clone
- Fn52
- Applications
- ELISA, WB, FuncS, RPE Cell Migration Assay, Fibronectin Assembly Assay, MMP Activity Assay
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western blot: 1/400-1/1000
FuncS: 10 μg/mL-25 μg/mL
Target
- Alternative Names
- FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF
- Gene ID
- 2335
- UniProt ID
- P02751
- Long Name
- Fibronectin 1
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1294z | Mouse Anti-FN1 Recombinant Antibody (clone 6H4) | IHC, IP, WB | Mouse IgG2b, κ |
| HPAB-0193-CN | Mouse Anti-FN1 Recombinant Antibody (HPAB-0193-CN) | ELISA, FC, IHC | Mouse IgG1 |
| HPAB-0194-CN | Mouse Anti-FN1 Recombinant Antibody (HPAB-0194-CN) | ELISA, FC, IHC | Mouse IgG1 |
| VS4-WK180 | Mouse Anti-FN1 Recombinant Antibody (VS4-WK180) | ELISA, WB | Mouse IgG |
| VS3-WK1577 | Mouse Anti-FN1 Recombinant Antibody (clone 6C10) | WB | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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