Mouse Anti-FN1 Recombinant Antibody (clone M9) (CAT#: ZG-0035C)
This product is a mouse antibody that recognizes FN1. It can be used in a wide variety of applications, such as: WB, IHC-P, IF/ICC.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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IHC
Figure 1 Mouse Anti-FN1 Antibody (clone M9) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, Fibronectin Monoclonal Antibody (M9) was diluted at 1:200 (4° C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98° C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IHC
Figure 2 Mouse Anti-FN1 Antibody (clone M9) in IHC
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Fibronectin Monoclonal Antibody (M9) was diluted at 1:200 (4° C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98° C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IF
Figure 3 Mouse Anti-FN1 Antibody (clone M9) in IF
Immunofluorescence analysis of Human-appendix tissue. 1, Fibronectin Monoclonal Antibody (M9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
IF
Figure 4 Mouse Anti-FN1 Antibody (clone M9) in IF
Immunofluorescence analysis of Mouse-spleen tissue. 1, Fibronectin Monoclonal Antibody (M9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
WB
Figure 5 Mouse Anti-FN1 Antibody (clone M9) in WB
WB analysis of Hela, diluted at 1:2000.
IHC
Figure 6 Mouse Anti-FN1 Antibody (clone M9) in IHC
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4° overnight). 2, High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 7 Mouse Anti-FN1 Antibody (clone M9) in IHC
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4° overnight). 2, High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 8 Mouse Anti-FN1 Antibody (clone M9) in IHC
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4° overnight). 2, High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
Specifications
- Immunogen
- Synthetic Peptide of Fibronectin
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Fibronectin
- Species Reactivity
- Human, Mouse, Rat
- Clone
- M9
- Applications
- WB, IHC-P, IF/ICC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence. For details, refer to Tested Data.
Target
- Alternative Names
- Fibronectin 1; Cold-Insoluble Globulin; Migration-Stimulating Factor; CIG; FN; Fibronectin; GFND2; SMDCF
- Gene ID
- 2335
- UniProt ID
- P02751
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-193 | Anti-Human Fibronectin Extra Domain-B Recombinant Antibody (Radretumab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | [(scFv - heavy - kappa) - IGHE - CH4]2 |
| TAB-001CQ-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (MOR03255) | ELISA, BL, IHC | Human antibody |
| TAB-002CQ-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (ME-4C) | ELISA, IHC | Human antibody |
| TAB-003CQ-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (1C5) | ELISA, WB | Human antibody |
| TAB-004CQ-F(E) | Human Anti-FN1 Recombinant Antibody; Fab Fragment (TAB-004CQ-F(E)) | ELISA, IHC, WB | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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