Mouse Anti-FN1 Recombinant Antibody (clone M9) (CAT#: ZG-0035C)

This product is a mouse antibody that recognizes FN1. It can be used in a wide variety of applications, such as: WB, IHC-P, IF/ICC.

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  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
IHC

Figure 1 Mouse Anti-FN1 Antibody (clone M9) in IHC

Figure 1 Mouse Anti-FN1 Antibody (clone M9) in IHC

Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, Fibronectin Monoclonal Antibody (M9) was diluted at 1:200 (4° C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98° C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.

IHC

Figure 2 Mouse Anti-FN1 Antibody (clone M9) in IHC

Figure 2 Mouse Anti-FN1 Antibody (clone M9) in IHC

Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Fibronectin Monoclonal Antibody (M9) was diluted at 1:200 (4° C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98° C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.

IF

Figure 3 Mouse Anti-FN1 Antibody (clone M9) in IF

Figure 3 Mouse Anti-FN1 Antibody (clone M9) in IF

Immunofluorescence analysis of Human-appendix tissue. 1, Fibronectin Monoclonal Antibody (M9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

IF

Figure 4 Mouse Anti-FN1 Antibody (clone M9) in IF

Figure 4 Mouse Anti-FN1 Antibody (clone M9) in IF

Immunofluorescence analysis of Mouse-spleen tissue. 1, Fibronectin Monoclonal Antibody (M9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

WB

Figure 5 Mouse Anti-FN1 Antibody (clone M9) in WB

Figure 5 Mouse Anti-FN1 Antibody (clone M9) in WB

WB analysis of Hela, diluted at 1:2000.

IHC

Figure 6 Mouse Anti-FN1 Antibody (clone M9) in IHC

Figure 6 Mouse Anti-FN1 Antibody (clone M9) in IHC

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4° overnight). 2, High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).

IHC

Figure 7 Mouse Anti-FN1 Antibody (clone M9) in IHC

Figure 7 Mouse Anti-FN1 Antibody (clone M9) in IHC

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4° overnight). 2, High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).

IHC

Figure 8 Mouse Anti-FN1 Antibody (clone M9) in IHC

Figure 8 Mouse Anti-FN1 Antibody (clone M9) in IHC

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4° overnight). 2, High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).


Specifications

  • Immunogen
  • Synthetic Peptide of Fibronectin
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Fibronectin
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • M9
  • Applications
  • WB, IHC-P, IF/ICC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Storage
  • Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence. For details, refer to Tested Data.

Target

  • Alternative Names
  • Fibronectin 1; Cold-Insoluble Globulin; Migration-Stimulating Factor; CIG; FN; Fibronectin; GFND2; SMDCF

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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