Rabbit Anti-FOLH1 Recombinant Antibody (clone R09-4F8)
CAT#: VS3-FY1859
This anti-PSMA antibody can be applied to western blot analysis of PSMA in 3T3 lysates. The recombinant monoclonal antibody exhibits high affinity for human and mouse FOLH1. The R09-4F8 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Gene Expression
Tested Data
Normal Tissue
Figure 1 Kidney
Proximal tubules (microvilli)
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1451/4354_A_7_5.jpg
Normal Tissue
Figure 2 Prostate
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1451/4354_A_2_5.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (HAP1, RPMI-8226, SK-BR-3, TIME, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000086205-FOLH1
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WB
Figure 1 Anti-FOLH1 Antibody (VS3-FY1859) in WB.
The PSMA expression in 3T3 lysates were detected using the anti-PSMA by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human PSMA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse FOLH1
- Species Reactivity
- Human, Mouse
- Clone
- R09-4F8
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 84 kDa, while the observed molecular weight stands at 100 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Folate Hydrolase 1; N-Acetylated-Alpha-Linked Acidic Dipeptidase I; Pteroylpoly-Gamma-Glutamate Carboxypeptidase; Folylpoly-Gamma-Glutamate Carboxypeptidase; Cell Growth-Inhibiting Gene 27 Protein; Membrane Glutamate Carboxypeptidase; Glutamate Carboxypeptidase II; Glutamate Carboxypeptidase 2; Glutamate Carboxylase II; EC 3.4.17.21; NAALADase I; NAALAD1; GCPII
- Gene ID
- 2346
- UniProt ID
- Q04609
- Sequence Similarities
- Belongs to the peptidase M28 family. M28B subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane
- Post Translation Modifications
- The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis. (Q04609-FOLH1_HUMAN)
The N-terminus is blocked. (Q04609-FOLH1_HUMAN)
Glycosylation at Asn76, Asn336, Asn51, Asn121, Asn140, Asn153, Asn195, Asn459, Asn476, and Asn638 (NX_Q04609 [NX_Q04609-1])
Modification sites at PhosphoSitePlus (Q04609)
Glycosylation from GlyGen (Q04609) 13 sites
- Protein Refseq
- XP_011518260.2; XP_016872921.1; XP_016872922.1; XP_016872923.1; XP_047282634.1
- Function
- Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| HPAB-0656-CN-S(P) | Mouse Anti-FOLH1 Recombinant Antibody; scFv Fragment (HPAB-0656-CN-S(P)) | WB, IF, FC | Mouse scFv |
| HPAB-1708-FY-F(E) | Human Anti-FOLH1 Recombinant Antibody; Fab Fragment (HPAB-1708-FY-F(E)) | Cyt | Human Fab |
| HPAB-1710-FY-F(E) | Human Anti-FOLH1 Recombinant Antibody; Fab Fragment (HPAB-1710-FY-F(E)) | Cyt | Human Fab |
| HPAB-J0213-YC-S(P) | Mouse Anti-FOLH1 Recombinant Antibody; scFv Fragment (HPAB-J0213-YC-S(P)) | ELISA, IHC, FuncS | Mouse scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0184CQ | Human Anti-FOLH1 Recombinant Antibody (HPAB-0184CQ) | ELISA, FC | Human IgG |
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|---|---|---|---|
| HPAB-0184CQ-F(E) | Human Anti-FOLH1 Recombinant Antibody Fab Fragment (HPAB-0184CQ-F(E)) | ELISA, FC | Human Fab |
| HPAB-0185CQ-F(E) | Human Anti-FOLH1 Recombinant Antibody (clone YC058); Fab Fragment | FC, IHC | Human Fab |
| HPAB-M0092-YC-F(E) | Mouse Anti-FOLH1 Recombinant Antibody Fab Fragment (HPAB-M0092-YC-F(E)) | FC | Mouse Fab |
| HPAB-1708-FY-S(P) | Human Anti-FOLH1 Recombinant Antibody; scFv Fragment (HPAB-1708-FY-S(P)) | Cyt | Human scFv |
| HPAB-1710-FY-S(P) | Human Anti-FOLH1 Recombinant Antibody; scFv Fragment (HPAB-1710-FY-S(P)) | Cyt | Human scFv |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY109 | Human Anti-FOLH1 (clone V5) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| VS-0425-YC356 | Recombinant Anti-FOLH1 Vesicular Antibody, EV Displayed (VS-0425-YC356) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
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| VS-0525-XY2603 | Anti-Human FOLH1 Immunohistochemistry Kit | IHC | |
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