Rabbit Anti-FOLH1 Recombinant Antibody (clone R09-4F8) (CAT#: VS3-FY1859)
This anti-PSMA antibody can be applied to western blot analysis of PSMA in 3T3 lysates. The recombinant monoclonal antibody exhibits high affinity for human and mouse FOLH1. The R09-4F8 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Specific Inquiry
WB
Figure 1 Anti-FOLH1 Antibody (VS3-FY1859) in WB.
The PSMA expression in 3T3 lysates were detected using the anti-PSMA by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human PSMA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse FOLH1
- Species Reactivity
- Human, Mouse
- Clone
- R09-4F8
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 84 kDa, while the observed molecular weight stands at 100 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Folate Hydrolase 1; N-Acetylated-Alpha-Linked Acidic Dipeptidase I; Pteroylpoly-Gamma-Glutamate Carboxypeptidase; Folylpoly-Gamma-Glutamate Carboxypeptidase; Cell Growth-Inhibiting Gene 27 Protein; Membrane Glutamate Carboxypeptidase; Glutamate Carboxypeptidase II; Glutamate Carboxypeptidase 2; Glutamate Carboxylase II; EC 3.4.17.21; NAALADase I; NAALAD1; GCPII
- Gene ID
- 2346
- UniProt ID
- Q04609
- Sequence Similarities
- Belongs to the peptidase M28 family. M28B subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane
- Post Translation Modifications
- The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis. (Q04609-FOLH1_HUMAN)
The N-terminus is blocked. (Q04609-FOLH1_HUMAN)
Glycosylation at Asn76, Asn336, Asn51, Asn121, Asn140, Asn153, Asn195, Asn459, Asn476, and Asn638 (NX_Q04609 [NX_Q04609-1])
Modification sites at PhosphoSitePlus (Q04609)
Glycosylation from GlyGen (Q04609) 13 sites
- Protein Refseq
- XP_011518260.2; XP_016872921.1; XP_016872922.1; XP_016872923.1; XP_047282634.1
- Function
- Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-066 | Recombinant Anti-Human FOLH1 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-277CT | Anti-Human PSMA Single Domain Antibody (TAB-277CT), Research Grade Biosimilar | ELISA | Single domain antibody |
| HPAB-M0386-YC | Recombinant Camelid Anti-FOLH1 Single Domain Antibody (HPAB-M0386-YC) | ELISA | Camelid VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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