Mouse Anti-FOXP1 Recombinant Antibody (clone 0725W35)
CAT#: VS-0725-FY196
This recombinant antibody, clone 0725W35, targets amino acids 495-578 of mouse FOXP1, capable of detecting three different FOXP1 isoforms. It is validated for Chromatin Immunoprecipitation (ChIP), Electrophoretic Mobility Shift Assay (EMSA), and Western Blot (WB). FOXP1 is a forkhead box transcription factor involved in tissue- and cell type-specific gene transcription, acting as a potential tumor suppressor. This antibody is a valuable tool for studying gene regulation, development, and cancer.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3876/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11501/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3876/11170_A_4_8_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11501/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11501/617_H10_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11501/27240_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endocrine cells
Staining:High
Intensity: Strong
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Fibroblasts
Staining:High
Intensity: Strong
Goblet cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11501/27240_A_9_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11501/27240_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11501/27240_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11501/27240_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced .
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000114861-FOXP1
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Specifications
- Immunogen
- Amino acids 495-578 of mouse FOXP1. Notably, this antibody can detect three different FOXP1 isoforms.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Mouse FOXP1
- Species Reactivity
- Mouse
- Clone
- 0725W35
- Applications
- Chromatin Immunoprecipitation, Electrophoretic Mobility Shift Assay, Western Blot
- Related Disease
- Neurodevelopmental disorders, Cancer, Autism spectrum disorder
Product Property
- Purification
- Protein A purified
- Molecular Weight
- The molecular weight of the antigen is 95, 70 & 50 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Specific conditions should be optimized by the end user.
Target
- Alternative Names
- Forkhead Box P1; Fork Head-Related Protein Like B; Mac-1-Regulated Forkhead; Glutamine-Rich Factor 1; MFH; PAX5/FOXP1 Fusion Protein
- Long Name
- Forkhead Box P1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Modification
Glycosylation from GlyGen 10 sites, 2 O-linked glycans (10 sites)
- Protein Refseq
- NP_001012523.1; NP_001231737.1; NP_001231739.1; NP_001231741.1; NP_001231742.1
- Function
- Acts cooperatively with FOXP4 to regulate lung secretory epithelial cell fate and regeneration by restricting the goblet cell lineage program; the function may involve regulation of AGR2.
Essential transcriptional regulator of B-cell development.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "FOXP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1070MZ | Recombinant Mouse Anti-Human FOXP1 Antibody | WB | Mouse antibody |
| MRO-0605-CN | Rabbit Anti-FOXP1 Recombinant Antibody (clone JF1017) | WB, IHC, FC | Rabbit IgG |
| VS3-XY725 | Mouse Anti-FOXP1 Recombinant Antibody (clone 6E4) | ELISA, WB, IHC, FC | Mouse IgG1 |
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| VS3-FY2712 | Rabbit Anti-FOXP1 Recombinant Antibody (VS3-FY2712) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1347 | Hi-Affi™ Rabbit Anti-FOXP1 Recombinant Antibody (clone DS1347AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY116 | CytoStream™ Mouse Anti-FOXP1 Recombinant Antibody (clone JC12) | FC | Mouse IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY861 | Anti-FOXP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2639 | Anti-Mouse FOXP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2638 | Anti-Human FOXP1 Immunohistochemistry Kit | IHC |
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