Mouse Anti-FSTL1 Recombinant Antibody (clone 22B6) (CAT#: HPAB-0165-WJ)

Figure 1 FSTL1-neutralizing antibody attenuates silica-induced lung inflammation and subsequent pulmonary fibrosis in mice.

(f) The level of cytokine IL-1β was detected by ELISA assay (*P < 0.05 by one-way ANOVA followed by Student's t test) for inflammation analysis.

Fang, Y., & Ning, W. (2016). Follistatin-like 1 aggravates silica-induced mouse lung injury and is a potential biomarker of chronic fibrotic lung diseases. Chest, 149(4).

Figure 2 FSTL1-neutralizing antibody attenuates silica-induced lung inflammation and subsequent pulmonary fibrosis in mice.

Representative images of the H&E staining of lung sections are shown (Scale bars, 200 μm) for fibrosis analysis.

Fang, Y., & Ning, W. (2016). Follistatin-like 1 aggravates silica-induced mouse lung injury and is a potential biomarker of chronic fibrotic lung diseases. Chest, 149(4).

Figure 3 FSTL1-neutralizing antibody attenuates TGF-β1 responsiveness in primary lung fibroblasts.

(A–C) Primary lung fibroblasts from C57BL/6J mice (n = 3; A and B) or from IPF patients (n = 2; C) were treated with 1 µg/ml FSTL1-neutralizing antibodies (22B6) or control IgG1 1 h before 5 ng/ml TGF-β1 treatment. (A and C) 24 h after TGF-β1 treatment, Western blot analysis of α-SMA in cell extracts (Cell), type I collagen (Col1), and fibronectin (Fn1) in the medium (supernatant [SN]) was performed. β-Actin was used as a loading control. Relative density of the bands of α-SMA normalized to β-actin is represented in the graphs. *, P < 0.05; **, P < 0.01.

Dong, Y., Geng, Y., Li, L., Li, X., Yan, X., Fang, Y., ... & Yang, X. (2015). Blocking follistatin-like 1 attenuates bleomycin-induced pulmonary fibrosis in mice. Journal of Experimental Medicine, 212(2), 235-252.

Figure 4 Specificity and neutralizing activity of FSTL1-neutralizing antibody.

(C and D) Kinetic analyses of the interaction of 22B6 mAb with FSTL1 (C) or Follistatin (D) by SPR. The y axis of the sensorgram is denoted as the RU signal, whereas the time (in seconds) is represented on the x axis. A series of concentrations of FSTL1 (C) or Follistatin (D) were injected at a flow rate of 10 µl/min.

Dong, Y., Geng, Y., Li, L., Li, X., Yan, X., Fang, Y., ... & Yang, X. (2015). Blocking follistatin-like 1 attenuates bleomycin-induced pulmonary fibrosis in mice. Journal of Experimental Medicine, 212(2), 235-252.

Figure 5 Specificity and neutralizing activity of FSTL1-neutralizing antibody.

(A and B) Primary lung fibroblasts from C57BL/6J mice (n = 3) were treated with 1 µg/ml anti-FSTL1 mAb (1F12 or 22B6) or control IgG1 1 h before 5 ng/ml TGF-1 treatment for 24 h. Col1a1 (A) and fibronectin (Fn1; B) mRNA expression were determined by qRT-PCR. *, P < 0.05.

Dong, Y., Geng, Y., Li, L., Li, X., Yan, X., Fang, Y., ... & Yang, X. (2015). Blocking follistatin-like 1 attenuates bleomycin-induced pulmonary fibrosis in mice. Journal of Experimental Medicine, 212(2), 235-252.