Recombinant Chicken Anti-Fusarium Antibody (CWP2) (CAT#: FAMAB-0116YC)

A chicken-derived single-chain antibody specific to antigens displayed on the Fusarium cell surface was isolated from a pooled immunocompetent phage display library. This recombinant antibody inhibited fungal growth in vitro when fused to any of the three AFPs.


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WB

Figure 1 Immunoblot of CWP from F. graminearum and F. oxysporum f.sp.matthiolae detected with the chicken-derived scFv antibody CWP2.

Figure 1 Immunoblot of CWP from F. graminearum and F. oxysporum f.sp.matthiolae detected with the chicken-derived scFv antibody CWP2.

Western blot analysis showed that the antibody bound to one protein with a molecular mass of approximately 50 kDa from cell wall preparations of both F. graminearum and F. oxysporum f.sp.matthiolae

Peschen, D., Li, H. P., Fischer, R., Kreuzaler, F., & Liao, Y. C. (2004). Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen. Nature biotechnology, 22(6), 732–738.

ELISA

Figure 2 Specificity and cross-reactivity of CWP2 to different Fusarium and non-Fusarium fungi determined by ELISA

Figure 2 Specificity and cross-reactivity of CWP2 to different Fusarium and non-Fusarium fungi determined by ELISA

High-binding ELISA plates were coated with germinated conidiospores from each Fusarium species, as described above. Colorimetric reactions were done upon adding p nitrophenyl phosphate substrate and measured at 405 nm.

Peschen, D., Li, H. P., Fischer, R., Kreuzaler, F., & Liao, Y. C. (2004). Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen. Nature biotechnology, 22(6), 732–738.

IF

Figure 3 Immunofluorescence labeling of germinated tubes of F. graminearum with scFv antibodies

Figure 3 Immunofluorescence labeling of germinated tubes of F. graminearum with scFv antibodies

The binding of CWP2 localized with the brightest, most continuous fluorescence intensity to the cell walls of germinated tubes, confirming the specificity of this antibody for a cell surface target.

Peschen, D., Li, H. P., Fischer, R., Kreuzaler, F., & Liao, Y. C. (2004). Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen. Nature biotechnology, 22(6), 732–738.


Specifications

  • Host Species
  • Chicken
  • Type
  • Chicken IgG
  • Specificity
  • Fusarium
  • Species Reactivity
  • Fusarium
  • Clone
  • CWP2
  • Applications
  • ELISA, Immunofluorescence
  • Related Disease
  • Fungal infection

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Storage
  • Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Target

  • Alternative Names
  • Fusarium; Filamentous fungi

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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