Mouse Anti-GAK Recombinant Antibody (VS-0224-XY51)
CAT#: VS-0224-XY51
The product is a mouse antibody against amino acids 1-360 of GAK of rat. It was purified by protein A. This product has been tested by Enzyme-linked immunosorbent assay, Western blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry-paraffin.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27463/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human caudate
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neurons.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27405/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27459/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27463/ihc_selected.jpg
Normal Tissue
Figure 5 Gallbladder
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27405/58449_A_5_4.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000178950-GAK
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- GAK
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ELISA, Western Blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry-paraffin
- Related Disease
- Prostate Signet Ring Cell Adenocarcinoma; Malignant Skin Fibrous Histiocytoma
Product Property
- Purification
- Protein A purified
- Buffer
- PBS
- Preservative
- 0.1% sodium azide and 0.1% gelatin
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested in ELISA (1/3000), Western blot (1/1000), Immunofluorescence (1/50-1/500), and Immunohistochemistry-paraffin (1/50-1/500).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "GAK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-084 | Recombinant Anti-Gak VHH Single Domain Antibody (PNBL-084) | ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2320CT | Recombinant Mouse anti-Human GAK Monoclonal antibody (FQS4537) | WB | |
| PABS-0276 | Camelid Anti-GAK Recombinant Antibody (clone 02J76) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY890 | Anti-GAK Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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