Human Anti-Gal/GalNAc Lectin Recombinant Antibody (clone CP33) (CAT#: HPAB-2040-FY)

This product is a recombinant human antibody that recognizes Gal/GalNAc Lectin. The monoclonal human antibodyCP33 reacts with the epitope of Gal/GalNAc Lectin.


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WB

Figure 1 Western immunoblot analysis of reactivities of human monoclonal antibody Fab fragments with trophozoites of E. histolytica HM-1:IMSS.

Figure 1 Western immunoblot analysis of reactivities of human monoclonal antibody Fab fragments with trophozoites of E. histolytica HM-1:IMSS.

The protein bands in lane 1 were stained with Coomassie brilliant blue. Lane 2, CP33; lane 3, CP33-H/L-CP17; lane 4, CP33-H/L-LA22; lane 5, CP33-H/L-CP26; lane 6, E.coli lysates (vector control); lane 7, plasma from a patient with an amebic liver abscess. The preparations in lanes 2 to 7 were treated with HRP-conjugated sheep antibody to human IgG F(ab )2.

Tachibana, H., Watanabe, K., Cheng, X. J., Tsukamoto, H., Kaneda, Y., Takeuchi, T., ... & Petri Jr, W. A. (2003). VH3 gene usage in neutralizing human antibodies specific for the Entamoeba histolytica Gal/GalNAc lectin heavy subunit. Infection and immunity, 71(8), 4313-4319.

DB

Figure 2 Dot immunoblot analysis of reactivities of human monoclonal antibody Fab fragments with an affinity-purified 260-kDa lectin and with rLecA.

Figure 2 Dot immunoblot analysis of reactivities of human monoclonal antibody Fab fragments with an affinity-purified 260-kDa lectin and with rLecA.

The spots were treated as follows: line 1, CP33; line 2, CP33-H/L-CP17; line 3, CP33-H/L-LA22; line 4, CP33-H/L-CP26; line 5, control human Fab; line 6, IgG purified from plasma of a patient with an amebic liver abscess; line 7, anti-260-kDa lectin rabbit antibody.

Tachibana, H., Watanabe, K., Cheng, X. J., Tsukamoto, H., Kaneda, Y., Takeuchi, T., ... & Petri Jr, W. A. (2003). VH3 gene usage in neutralizing human antibodies specific for the Entamoeba histolytica Gal/GalNAc lectin heavy subunit. Infection and immunity, 71(8), 4313-4319.


Specifications

  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • E. histolytica Gal/GalNAc Lectin
  • Species Reactivity
  • E. histolytica
  • Clone
  • CP33
  • Applications
  • WB, DB
  • Related Disease
  • E. histolytica

Product Property

  • Purity
  • >95% as determined by SDS-PAGE analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The Gal/GalNAc Lectin antibody has been reported in applications of Western Blot, Dot Blot.

Target

  • Alternative Names
  • E. histolytica Gal/GalNAc lectin; Gal/GalNAc Lectin

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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scFv Fragment Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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