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  • Human Anti-GAPDH Recombinant Antibody (clone lesion); scFv Fragment

Human Anti-GAPDH Recombinant Antibody (clone lesion); scFv Fragment (CAT#: FAMAB-0117-YC-S(P))

GAPDH is glycolytic enzymes essential for efficient energy production and are expressed in all prokaryotic and eukaryotic organisms. In addition to its glycolytic functions, GAPDH is a multifunctional protein endowed with diverse activities, including phosphorylating transverse-tubule proteins, stimulating RNA transcription, and influencing DNA replication and DNA repair. TPI and GAPDH have been considered to associate with brain microtubules. Anti-TPI or anti-GAPDH Abs may induce an immune hypersensitivity reaction, through activation of complements and macrophages, leading to neuronal and axonal degeneration in MS.


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Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/752_B3_1_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/if_selected.jpg
Figure 7 IF staining of human cell line A549
Figure 7 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/1874_F7_61_blue_red_green.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach, lower shows strong nuclear and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/ihc_selected.jpg
Figure 4 IHC staining of human skin
Figure 4 IHC staining of human skin
(Immunohistochemical staining of human skin shows strong nuclear positivity in keratinocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_B_8_1_selected.jpg
Figure 5 IHC staining of human kidney
Figure 5 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows cytoplasmic and nuclear positivity in cells in tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5197/ihc_selected.jpg
Figure 6 IHC staining of human skeletal muscle
Figure 6 IHC staining of human skeletal muscle
(Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79968/170623_B_3_6_selected.jpg
Figure 8 Colon
Figure 8 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_3.jpg
Figure 9 Liver
Figure 9 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_4.jpg
Figure 10 Kidney
Figure 10 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_5.jpg
Figure 11 Testis
Figure 11 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_4_6.jpg
Figure 12 Lymph node
Figure 12 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_8.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Figure 13 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111640-GAPDH

Specifications

  • Host Species
  • Human
  • Type
  • Human scFv
  • Specificity
  • Human GAPDH
  • Species Reactivity
  • Human
  • Clone
  • lesion
  • Applications
  • ELISA, WB
  • Related Disease
  • Multiple sclerosis

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • G3PD; GAPD; HEL-S-162eP

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

CAT Product Name Application Type
MOB-386 Recombinant Anti-human GAPDH Antibody WB, IHC, FuncS IgG
MRO-1883-CN Rabbit Anti-GAPDH Polyclonal Antibody (MRO-1883-CN) WB, IF, IHC, FC Rabbit IgG
MRO-1884-CN Rabbit Anti-GAPDH Polyclonal Antibody (MRO-1884-CN) WB, IF, IHC, FC Rabbit IgG
MRO-1885-CN Rabbit Anti-GAPDH Polyclonal Antibody (MRO-1885-CN) WB, IF, IHC, FC Rabbit IgG
ZG-0053C Mouse Anti-GAPDH Recombinant Antibody (clone 2B8-1) WB Mouse IgG

Fab Fragment Antibody

CAT Product Name Application Type
MOB-386-F(E) Recombinant Anti-human GAPDH Antibody Fab Fragment IP, RIA, FuncS Fab
MHH-386-F(E) Recombinant Human Anti-human GAPDH Antibody Fab Fragment ELISA, WB, IP, FuncS Fab
FAMAB-0117-YC-F(E) Human Anti-GAPDH Recombinant Antibody (clone lesion); Fab Fragment ELISA, WB Human Fab

scFv Fragment Antibody

CAT Product Name Application Type
MHH-386-S(P) Recombinant Human Anti-human GAPDH Antibody scFv Fragment WB, FC, FuncS scFv

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0217MZ Chicken Anti-GAPDH Polyclonal IgY WB Chicken antibody
BRD-0699MZ Chicken Anti-GAPDH Polyclonal IgY WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1398 Hi-Affi™ Rabbit Anti-GAPDH Recombinant Antibody (clone DS1398AB) WB, ICC, IP, FC Rabbit IgG
MOR-0101-FY Rabbit Anti-GAPDH Recombinant Antibody (clone AFY0072) IF, IHC-P, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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