Mouse Anti-GAPDH Recombinant Antibody (clone 13B6) (CAT#: VS4-WK190)
This product is a mouse IgG antibody that recognizes human GAPDH. The 13B6 antibody is recommended for WB (dilution range 1/500-1/5000).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/752_B3_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/752_B3_1_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/if_selected.jpg
Figure 7 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/1874_F7_61_blue_red_green.jpg
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/1874_F7_61_blue_red_green.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach, lower shows strong nuclear and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/ihc_selected.jpg
(Immunohistochemical staining of human stomach, lower shows strong nuclear and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/ihc_selected.jpg
Figure 4 IHC staining of human skin
(Immunohistochemical staining of human skin shows strong nuclear positivity in keratinocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_B_8_1_selected.jpg
(Immunohistochemical staining of human skin shows strong nuclear positivity in keratinocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_B_8_1_selected.jpg
Figure 5 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows cytoplasmic and nuclear positivity in cells in tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5197/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows cytoplasmic and nuclear positivity in cells in tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5197/ihc_selected.jpg
Figure 6 IHC staining of human skeletal muscle
(Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79968/170623_B_3_6_selected.jpg
(Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79968/170623_B_3_6_selected.jpg
Figure 8 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_3.jpg
Figure 9 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_4.jpg
Figure 10 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_5.jpg
Figure 11 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_4_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_4_6.jpg
Figure 12 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_8.jpg
Figure 13 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111640-GAPDH
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111640-GAPDH
Specifications
- Immunogen
- Synthetic Peptide
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human GAPDH
- Species Reactivity
- Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep
- Clone
- 13B6
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- The antibody was purified from mouse ascites by immunogen affinity chromatography.
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS containing 50% glycerol, pH 7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at -20°C or -80°C. Avoid repeated freeze.
- Shipping
- Ice packs
Applications
- Application Notes
- Recommended dilution: WB:1:500-1:5000
Target
- Alternative Names
- Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase, 38 Kd Component; Aging-Associated Gene 9 Protein;
- Gene ID
- 2597
- UniProt ID
- P04406
- Long Name
- Glyceraldehyde-3-Phosphate Dehydrogenase
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-386-F(E) | Recombinant Anti-human GAPDH Antibody Fab Fragment | IP, RIA, FuncS | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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