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    Rabbit Anti-GAPDH Recombinant Antibody (clone AFY0072) (CAT#: MOR-0101-FY)

    This product is a recombinant Rabbit antibody that recognizes GAPDH. The monoclonal Rabbit antibody AFY0072 reacts with the epitope (c-terminal) of GAPDH.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/752_B3_1_selected.jpg
    Figure 2 IF staining of human cell line U-2 OS
    Figure 2 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/if_selected.jpg
    Figure 7 IF staining of human cell line A549
    Figure 7 IF staining of human cell line A549
    (Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/1874_F7_61_blue_red_green.jpg
    Normal Tissue
    Figure 3 IHC staining of human stomach
    Figure 3 IHC staining of human stomach
    (Immunohistochemical staining of human stomach, lower shows strong nuclear and cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/ihc_selected.jpg
    Figure 4 IHC staining of human skin
    Figure 4 IHC staining of human skin
    (Immunohistochemical staining of human skin shows strong nuclear positivity in keratinocytes.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_B_8_1_selected.jpg
    Figure 5 IHC staining of human kidney
    Figure 5 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows cytoplasmic and nuclear positivity in cells in tubules.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5197/ihc_selected.jpg
    Figure 6 IHC staining of human skeletal muscle
    Figure 6 IHC staining of human skeletal muscle
    (Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/79968/170623_B_3_6_selected.jpg
    Figure 8 Colon
    Figure 8 Colon
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_3.jpg
    Figure 9 Liver
    Figure 9 Liver
    (Hepatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_4.jpg
    Figure 10 Kidney
    Figure 10 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Collecting ducts
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Distal tubules
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Proximal tubules (cell body)
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_5.jpg
    Figure 11 Testis
    Figure 11 Testis
    (Cells in seminiferous ducts
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_4_6.jpg
    Figure 12 Lymph node
    Figure 12 Lymph node
    (Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_8.jpg
    RNA Expression
    Figure 13 RNA cell line category: Low cell line specificity
    Figure 13 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111640-GAPDH

    Specifications

    • Immunogen
    • C-terminal half of human Glyceraldehyde-3-phosphate dehydrogenase.
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human GAPDH
    • Species Reactivity
    • Human
    • Clone
    • AFY0072
    • Applications
    • IF, IHC-P, WB
    • Conjugate
    • Unconjugated

    Product Property

    • Purity
    • >95% as determined by analysis by SDS-PAGE
    • Format
    • Lyophilized
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS, 5% Trehalose
    • Preservative
    • No preservatives
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • This antibody has been tested for use in Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot.

    Target

    • Alternative Names
    • Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase, 38 Kd Component; Aging-Associated Gene 9 Protein

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "GAPDH"

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    CAT Product Name Application Type
    MOB-386-F(E) Recombinant Anti-human GAPDH Antibody Fab Fragment IP, RIA, FuncS Fab
    MHH-386-F(E) Recombinant Human Anti-human GAPDH Antibody Fab Fragment ELISA, WB, IP, FuncS Fab
    FAMAB-0117-YC-F(E) Human Anti-GAPDH Recombinant Antibody (clone lesion); Fab Fragment ELISA, WB Human Fab

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