CytoStream™ Mouse Anti-GFAP Recombinant Antibody (VS13-YC444)
CAT#: VS13-YC444
The CytoStream™ Mouse Anti-GFAP Recombinant Antibody is specific for human, mouse and rat GFAP. GFAP, a key astrocyte intermediate filament, distinguishes glial cells and is involved in Alexander disease through mutations affecting structural stability and CNS interactions. The anti-GFAP antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1475_F11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1326_A1_4_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/135460_B_9_5.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
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Specifications
- Immunogen
- Recombinant human GFAP.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse and Rat GFAP
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
- Related Disease
- Alexander disease
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 40% Glycerol and 0.2% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50
Immunofluorescence (IF): 1/50-1/500
Immunohistochemistry-paraffin (IHC-P): 1/200-1/600
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Glial Fibrillary Acidic Protein; Intermediate Filament Protein; ALXDRD;
- Gene ID
- 2670
- UniProt ID
- P14136
- Long Name
- Glial Fibrillary Acidic Protein
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm; Intermediate filament
- Protein Refseq
- NP_001124491.1; NP_001229305.1; NP_002046.1
- Function
- GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "GFAP"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-068 | Recombinant Anti-Mouse Gfap VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-083CQ | Llama Anti-GFAP Recombinant Antibody (TAB-083CQ) | IHC, WB, IF | Llama VHH |
| TAB-084CQ | Llama Anti-GFAP Recombinant Antibody (TAB-084CQ) | WB | Llama VHH |
| HPAB-1158-FY | Recombinant Anti-GFAP Single Domain Antibody (HPAB-1158-FY) | WB, ELISA, IHC | Llama VHH |
| HPAB-1159-FY | Recombinant Anti-GFAP Single Domain Antibody (HPAB-1159-FY) | WB, ELISA, IHC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0805MZ | Chicken Anti-GFAP Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1122MZ | Chicken Anti-GFAP Polyclonal IgY | IHC, IF, WB | Chicken antibody |
| BRD-1174MZ | Chicken Anti-Glial Fibrillary Acidic Protein (GFAP) Polyclonal IgY | WB, ICC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1426 | Hi-Affi™ Rabbit Anti-GFAP Recombinant Antibody (clone DS1426AB) | ICC, IHC-Fr, IHC-P, IP, WB | Rabbit IgG |
| MOR-0100-FY | Rabbit Anti-GFAP Recombinant Antibody (clone AFY0071) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0648-CN | Rabbit Anti-GFAP Recombinant Antibody (clone CBACN-238) | WB, IHC, IP | Rabbit IgG |
| MRO-0649-CN | Mouse Anti-GFAP Recombinant Antibody (clone 1-D4) | WB, IHC, IF, FC | Mouse IgG1 |
| ZG-1002F | Mouse Anti-GFAP Recombinant Antibody (clone 6F2) | IHC-Fr, IHC-P, WB | Mouse IgG1 |
| VS4-WK195 | Mouse Anti-GFAP Recombinant Antibody (VS4-WK195) | ELISA, WB | Mouse IgG |
| VS11-QX73 | Mouse Anti-GFAP Recombinant Antibody (clone 10G1G11H7) | WB, ELISA, IHC | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0197-WJ-S(P) | Mouse Anti-GFAP Recombinant Antibody (clone 5C8); scFv Fragment | ELISA, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0197-WJ-F(E) | Mouse Anti-GFAP Recombinant Antibody (clone 5C8); Fab Fragment | ELISA, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC176 | AbPlus™ Anti-GFAP Magnetic Beads (VS-0724-YC176) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY912 | Anti-GFAP Immunohistochemistry Kit | IHC | |
| VS-0525-XY2784 | Anti-Mouse GFAP Immunohistochemistry Kit | IHC | |
| VS-0525-XY2783 | Anti-Human GFAP Immunohistochemistry Kit | IHC | |
| VS-0525-XY2785 | Anti-Porcine GFAP Immunohistochemistry Kit | IHC |
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