CytoStream™ Mouse Anti-GFAP Recombinant Antibody (VS13-YC444) (CAT#: VS13-YC444)
The CytoStream™ Mouse Anti-GFAP Recombinant Antibody is specific for human, mouse and rat GFAP. GFAP, a key astrocyte intermediate filament, distinguishes glial cells and is involved in Alexander disease through mutations affecting structural stability and CNS interactions. The anti-GFAP antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1475_F11_2_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1475_F11_2_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1326_A1_4_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1326_A1_4_blue_red_green.jpg
Figure 3 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/135460_B_9_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/135460_B_9_5.jpg
Figure 4 RNA cell line category: Cell line enhanced (U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
Figure 4 RNA cell line category: Cell line enhanced (U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
Specifications
- Immunogen
- Recombinant human GFAP.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse and Rat GFAP
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
- Related Disease
- Alexander disease
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 40% Glycerol and 0.2% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50
Immunofluorescence (IF): 1/50-1/500
Immunohistochemistry-paraffin (IHC-P): 1/200-1/600
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Glial Fibrillary Acidic Protein; Intermediate Filament Protein; ALXDRD;
- Gene ID
- 2670
- UniProt ID
- P14136
- Long Name
- Glial Fibrillary Acidic Protein
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm; Intermediate filament
- Protein Refseq
- NP_001124491.1; NP_001229305.1; NP_002046.1
- Function
- GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-068 | Recombinant Anti-Mouse Gfap VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-083CQ | Llama Anti-GFAP Recombinant Antibody (TAB-083CQ) | IHC, WB, IF | Llama VHH |
| TAB-084CQ | Llama Anti-GFAP Recombinant Antibody (TAB-084CQ) | WB | Llama VHH |
| HPAB-1158-FY | Recombinant Anti-GFAP Single Domain Antibody (HPAB-1158-FY) | WB, ELISA, IHC | Llama VHH |
| HPAB-1159-FY | Recombinant Anti-GFAP Single Domain Antibody (HPAB-1159-FY) | WB, ELISA, IHC | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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