Recombinant Anti-GFAP Single Domain Antibody (HPAB-1158-FY) (CAT#: HPAB-1158-FY)
This product is a recombinant Single Domain Antibody that recognizes GFAP. The monoclonal Single Domain Antibody E3 reacts with the epitope of GFAP.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1475_F11_2_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1475_F11_2_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1326_A1_4_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1326_A1_4_blue_red_green.jpg
Figure 3 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/135460_B_9_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/135460_B_9_5.jpg
Figure 4 RNA cell line category: Cell line enhanced (U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
Figure 4 RNA cell line category: Cell line enhanced (U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
Specifications
- Host Species
- Llama
- Type
- Llama VHH
- Specificity
- Human GFAP
- Species Reactivity
- Human
- Clone
- HPAB-1158-FY
- Applications
- WB, ELISA, IHC
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The GFAP antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
WB: following the separation by polyacrylamide gel electrophoresis (PAGE) using NuPAGE Novex 4–12% Bis–Tris gel, semi-dry transfer onto Hybond-C and Western blotting were carried out using the Xcell II blot module. Before the immunochemical reaction, membranes were blocked in a 4% skimmed milk solution. Immunoblotting of membranes was accomplished with VHH (VHH A10 1 μg/ml, VHH E9 and VHH E3 0.1 μg/ml), and revealed by anti-His-tag 1:1,000 followed by peroxidaselabeled goat anti-rabbit immunoglobulins 1:10,000. Finally, peroxidase activity was visualized using a chemiluminescent kit.
Target
- Alternative Names
- Glial Fibrillary Acidic Protein; Intermediate Filament Protein; ALXDRD
- Gene ID
- 2670
- UniProt ID
- P14136
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-068 | Recombinant Anti-Mouse Gfap VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-083CQ | Llama Anti-GFAP Recombinant Antibody (TAB-083CQ) | IHC, WB, IF | Llama VHH |
| TAB-084CQ | Llama Anti-GFAP Recombinant Antibody (TAB-084CQ) | WB | Llama VHH |
| HPAB-1159-FY | Recombinant Anti-GFAP Single Domain Antibody (HPAB-1159-FY) | WB, ELISA, IHC | Llama VHH |
Customer Reviews and Q&As
Customer Review
Q&As
Works Well in WB
We have used recombinant anti-GFAP single domain antibody (HPAB-1158-FY), and it gave nice results by WB. We used 1:1000 for WB, and it detected GFAP tagged protein very clear without unspecific band. This antibody has been widely used in our lab.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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