Mouse Anti-GFP Recombinant Antibody (clone 6C11C11) (CAT#: ZG-0293U)

Figure 1 Mouse Anti-GFP Antibody (ZG-0293U) in WB

Positive WB detected in: 1-4 lanes: Recombinant proteins with GFP tag for 50ng; 5 lane: 293F whole cell lysate transfected with GFP for 5μg All lanes GFP antibody at 1: 5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 1,2,3,4 and 5 is 32,32,50,32,32 KDa respectively
Observed band size: 32,32,50,32,32 KDa
Exposure time: 1min

Figure 2 Mouse Anti-GFP Antibody (ZG-0293U) in IF

Immunofluorescence staining of 293F cells transfected with GFP with ZG-0293U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).

Figure 3 Mouse Anti-GFP Antibody (ZG-0293U) in FC

Overlay histogram showing 293F cells transfected with GFP stained with ZG-0293U (red line) at 1:200. The cells were fixed in 70% ethanol at 4°C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Figure 4 Mouse Anti-GFP Antibody (ZG-0293U) in IP

Immunoprecipitating GFP in 293F whole cell lysate transfected with GFP. Lane 1: Mouse control IgG2b in 293F whole cell lysate transfected with GFP
Lane 2: ZG-0293U (4µg) + 293F whole cell lysate transfected with GFP (500µg)
Lane 3: 293F whole cell lysate transfected with GFP (5µg)
For western blotting, the blot was detected with ZG-0293U at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000