The antibody LaG-16 is an anti-GFP VHH sdAb which binds to GFP with high affinity. This is a high affinity sdAb directly obtained from animal serum.
Figure 1 Efficacy of LaG sdAb in immunofluorescence microscopy.
HeLa cells transiently transfected with tubulin-emGFP marker were fixed and immunostained with LaG-16 conjugated to Alexa Fluor® 568 (AF568). Cells were visualized in the red channels, with DAPI counter-staining of nuclei (blue).
Fridy, P. C., Li, Y., Keegan, S., Thompson, M. K., Nudelman, I., Scheid, J. F., ... & Rout, M. P. (2014). A robust pipeline for rapid production of versatile sdAb repertoires. Nature methods, 11(12), 1253.
Figure 2 sdAb fluorescent protein binding.
Thirteen high-affinity LaGs were conjugated to magnetic beads and incubated with various recombinant fluorescent proteins. All LaGs bound A. victoria GFP variants.
Fridy, P. C., Li, Y., Keegan, S., Thompson, M. K., Nudelman, I., Scheid, J. F., ... & Rout, M. P. (2014). A robust pipeline for rapid production of versatile sdAb repertoires. Nature methods, 11(12), 1253.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
PFBZ-050 | Mouse Anti-GFP Recombinant Antibody (clone 11G10); Fab Fragment | ELISA, WB | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBZ-050 | Mouse Anti-GFP Recombinant Antibody (clone 11G10); scFv Fragment | ELISA, WB | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1428 | Hi-Affi™ Rabbit Anti-GFP Recombinant Antibody (clone DS1428AB) | WB | Rabbit IgG |
MOR-0061-FY | Rabbit Anti-GFP Recombinant Antibody (clone AFY0032) | ICC, IF, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-1891-CN | Rabbit Anti-GFP Polyclonal Antibody (MRO-1891-CN) | WB, ELISA | Rabbit IgG |
VS3-WK1092 | Mouse Anti-GFP Recombinant Antibody (VS3-WK1092) | WB, IHC, ICC, IF, IP | Mouse IgG1 |
VS3-WK1556 | Mouse Anti-GFP Recombinant Antibody (clone 2G6) | WB | Mouse IgG1 |
VS3-WK1559 | Mouse Anti-GFP Recombinant Antibody (clone 152) | WB, IP | Mouse IgG1 |
VS3-WK1562 | Mouse Anti-GFP Recombinant Antibody (clone 156) | WB, IP | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-AP896-YC | Recombinant Camel Anti-GFP Single Domain Antibody (2A12) | ELISA, WB | Camel VHH |
HPAB-AP897-YC | Recombinant Camel Anti-GFP Single Domain Antibody (2G2) | ELISA, WB | Camel VHH |
HPAB-AP898-YC | Recombinant Camel Anti-GFP Single Domain Antibody (2G9) | ELISA, WB | Camel VHH |
HPAB-AP899-YC | Recombinant Camel Anti-GFP Single Domain Antibody (3H8) | ELISA, WB | Camel VHH |
HPAB-AP900-YC | Recombinant Camel Anti-GFP Single Domain Antibody (7C8) | ELISA, WB | Camel VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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